Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction |
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| Authors: | Laura Selva,Cristina Esteva,Amadeu Gené ,Maria Ferná ndez de Sevilla,Susanna Hernandez-Bou,Carmen Muñ oz-Almagro |
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| Affiliation: | 1. Molecular Microbiology Department, University Hospital Sant Joan de Déu, 08950 Esplugues-Barcelona, Spain;2. Paediatrics Department, University Hospital Sant Joan de Déu, Esplugues-Barcelona, Spain |
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| Abstract: | We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen. |
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| Keywords: | Real-time PCR Blood culture bottles Streptococcus pneumoniae |
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