2005—2007年河北省水产品副溶血性弧菌主动监测及耐药性研究

目的了解河北省水产品中副溶血弧菌（Vibrio Parahaemolyticus，VP）的污染状况及耐药性的变化，为建立VP食源性疾病的预警提供数据基础。方法依据GB／T4789．7—2003对河北省各类水产品进行VP分离，用API20E进行生化鉴定，同时用纸片法进行耐药性监测。结果363份样品检出108株副溶血性弧菌，检出率29，8％，2005—2007年检出率逐年降低，统计学有显著性差异（x^2=44．42，P〈0．01）。分离菌株对诺氟沙星和氯霉素100％敏感，对氨苄西林、链霉素、磺胺、阿米卡星显示高度耐药，对氨苄西林的耐药率高达94．4％。结论河北省水产品VP污染严重，继续加强VP食源性疾病预警，同时加强水产品抗生素使用的管理，防止耐药菌株的传播。

Surveillance and Drug Resistance Analysis on Vibrio Parahaemolyticus in Aquatic Products in Hebei from 2005 to 2007

Objective To investigate the contaminatron status and drug resistance of Vibdo parahaemolyticus （VP） in aquatic products in Hebei Province, to offer data for establishing early- warnrng system of food poisoning caused by VP. Methods The VP from aquatic products in Hebei Provrnce was separated according to GB/T4789.7 - 2003, and identified by API 20E. K - B method was used to perform mfcrobral sensitivity. Results A total of 108 of 363 samples were positive for VP （29.8%）. The positive rate decreased year by year during the period of 2005 - 2007 , there showed statistically significant difference （x^2 =44.42, P〈 0.01）. All the strains showed sensitivity to norfloxacin and chloramphenicol, but most strains showed resistance to ampicillin, streptomycin, sulfonamides, and amikacin. The drug resistance rate to ampicillin was 94.4%. Conclusions The contamination situation of VP in aquatic products in Hebei is rather severe. For preventing the VP spread, it is necessary te constantly strengthen early- warning system of food poisoning caused by VP, and enhance the management of antibiotics use.