Detection of Haemophilus influenzae type b by real-time PCR |
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| Authors: | Marty Adrian Greiner Oliver Day Philip J R Gunziger Sibylle Mühlemann Kathrin Nadal David |
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| Affiliation: | Division of Infectious Diseases, University Children's Hospital of Zurich, Steinwiesstr. 75, CH-8032 Zurich, Switzerland. |
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| Abstract: | A real-time PCR assay targeting the capsulation locus of Haemophilus influenzae type b (Hib) was developed. The linear detection range was from 1 to 10(6) microorganisms per reaction mixture. No H. influenzae other than Hib or any other control bacteria typically found in the upper respiratory tract was detected. |
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