G-CSF对肌萎缩侧索硬化细胞外谷氨酸摄取和抗自由基作用的影响

目的研究在肌萎缩侧索硬化的体外模型中,粒细胞集落刺激因子(G-CSF)是否可以通过促进细胞外谷氨酸摄取和抗自由基作用发挥对运动神经元的保护作用。方法取8日龄SD乳鼠断头处死,取脊髓切片后放入培养液中构建脊髓器官模型。将构建的脊髓器官模型随机分为对照组、肌萎缩侧索硬化症(ALS)模型组和G-CSF组。对照组不做任何处理,仍常规培养;ALS模型组按照Matyja等人的方法加入THA和PDC构建ALS模型;G-CSF组在ALS模型组处理的基础上再加入重组人粒细胞集落刺激因子。在培养的1w、2w、3w、4w时对各组培养液中的Glu进行测定,并在第4w测定培养液中超氧化物歧化酶(SOD)、丙二醛(MDA)含量,免疫组化染色计数各组前角存活的运动神经元。结果对照组培养液中Glu浓度较低,并且随时间变化(1w、2w、3w、4w)不大,基本维持在恒定水平;ALS模型组和G-CSF组培养液中Glu浓度随时间延长逐渐增加,2w后G-CSF组Glu浓度显著低于ALS组(P0.05)。第4周时ALS模型组脊髓前角中SOD活性显著低于对照组(P0.05),G-CSF组SOD活性高于ALS模型组(P0.05),但仍低于对照组(P0.05)。结论 G-CSF有显著的抗自由基和抗兴奋性毒性作用并明显减少脊髓前角运动神经元的凋亡和缺失,可能为肌萎缩侧索硬化提供新的治疗方向。

Effect of G-CSF on amyotrophic lateral sclerosis extracellular glutamate uptake and anti-free radicals effect

Objective To study the protection of G-CSF on the motor neurons by promoting extracellular glutamate uptake and anti-free radicals effect in vitro models of amyotrophic lateral sclerosis(ALS).Methods 8-day-old neonatal SD rats were decapitated,and then cultured the spinal cord slices in medium to organize the organ model.The models were randomly divided into control group,ALS model group and G-CSF group.The control group maintained a normal culture without any treatment.ALS model group was treated with THA ...