| 黄体酮对局灶性脑缺血再灌注大鼠水通道蛋白-4表达及血脑屏障通透性的影响 |
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| 引用本文： | 赵春艳,王建平,赵源征,朱中君. 黄体酮对局灶性脑缺血再灌注大鼠水通道蛋白-4表达及血脑屏障通透性的影响[J]. 中风与神经疾病杂志, 2010, 27(11) |
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| 作者姓名： | 赵春艳 王建平 赵源征 朱中君 |
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| 作者单位： | 郑州大学第五附属医院神经内科,河南,郑州,450052 |
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| 摘 要： | 目的研究黄体酮对大鼠局灶性脑缺血再灌注后脑组织内水通道蛋白-4(AQP4)的表达及血脑屏障通透性的影响。方法健康雄性SD大鼠96只,随机分为4大组:假手术组、手术组、溶剂治疗组和黄体酮治疗组,建立大脑中动脉栓塞再灌注(MCAO/R)模型,分别在缺血2h再灌注6h、1d、3d、5d 4个时间点将大鼠麻醉后断头取脑,采用Western blot法、伊文氏蓝(EB)渗出量及干湿重法分别测定脑组织AQP4的表达、血脑屏障的通透性及脑含水量。结果手术组AQP4的表达、EB的含量及脑组织含水量明显高于假手术组;黄体酮组AQP4的表达、EB的含量及脑组织含水量较手术组及溶剂组明显降低,差异有统计学意义。结论黄体酮可以降低缺血再灌注大鼠脑组织AQP4的表达,从而降低血脑屏障的通透性,减轻脑水肿。
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| 关 键 词： | 黄体酮 水通道蛋白-4 脑缺血再灌注 血脑屏障 脑含水量 |
Effect of progesterone on expression of aquaporin-4 and permeability of blood brain barrier after focal brain ischemia-reperfusion in rats |
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| ZHAO Chun-yan,WANG Jian-ping,ZHAO Yuan-zheng,et al.. Effect of progesterone on expression of aquaporin-4 and permeability of blood brain barrier after focal brain ischemia-reperfusion in rats[J]. Journal of Apoplexy and Nervous Diseases, 2010, 27(11) |
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| Authors: | ZHAO Chun-yan WANG Jian-ping ZHAO Yuan-zheng et al. |
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| Affiliation: | ZHAO Chun-yan,WANG Jian-ping,ZHAO Yuan-zheng,et al.(Department of Neurology,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China) |
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| Abstract: | Objective To investigate the effects of progesterone on expression of aquaporin-4(AQP4)and permeability of blood brain barrier(BBB)after focal brain ischemia-reperfusion in rats.Methods Ninety-six healthy male SD rats were randomly divided into sham surgery group,surgery group,vehicle-treated group and progesterone-treated group.The model of focal brain ischemia-reperfusion injury was induced by middle cerebral artery occlusion(MCAO).After 2h temporary MCAO,rats were respectively subjected to reperfusion of... |
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| Keywords: | Progesterone Aquaporin-4 Brain ischemia-reperfusion Blood brain barrier Brain water content |
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