| 5-氟尿嘧啶对人胃癌细胞超微结构和GSTπ表达的影响 |
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| 引用本文： | 张江兰,;薄爱华,;吴靖芳,;任君旭.5-氟尿嘧啶对人胃癌细胞超微结构和GSTπ表达的影响[J].张家口医学院学报,2008(3):17-18. |
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| 作者姓名： | 张江兰 ;薄爱华 ;吴靖芳 ;任君旭 |
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| 作者单位： | [1]河北北方学院医学院组织胚胎学教研室,河北张家口075000; [2]河北北方学院实验中心电镜室,河北张家口075000 |
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| 摘 要： | 目的：探讨5-氟尿嘧啶对人胃癌细胞的结构和GSTπ表达的影响。方法：在人胃癌BGC-823细胞株培养液中加入5-氟尿嘧啶,继续培养5h或10h,然后用HE、免疫组化染色和铀-铅染色。光镜、电镜下观察。结果：10h组胃癌细胞外型畸变、细胞器破坏严重;GSTπ灰度发生变化。结论：5-氟尿嘧啶作用10h对人胃癌细胞超微结构及GSTπ基因表达有明显影响。
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| 关 键 词： | 胃肿瘤 免疫组织化学 5-氟尿嘧啶 |
Effects of 5-Fliorouracil on the Ultrastructure and the Expressions of GSTπ in Human Gastric Cancer Cells |
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| Institution: | ZHANG Jiang-lan, BO Ai-hua,WU Jing-fang, et al(Hebei North University,Zhangjiakou,075000, Hebei,China) |
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| Abstract: | Objective：To study the influence of 5-Fliorouracil on structure of human gastric cancer cells and the expressions of GSTπ. Methods： 5-Fliorouracil was added to culture medium of BGC-823 gastric cancer cells and continuously cultured for 5h or 10h,then the human gastric cancer cells were stained with haematoxylin- Eosin（HE）,dyed with immunohistochemical method and acid U-lead citrate, which was observed under light and electiom microscope. Results： BGC-823 cells, features developed by aberration and the oraganells were destructed severely in experimental group of 10 hours, and the ash of GSTπ was changed. Conclusion：The influence of 5-Fliorouracil on ultrastructure and the expressions of GSTπ in 10 hours group was obvious. |
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| Keywords: | Stomach Neoplasms Immunohistochemistry Research 5-Fliorouracil |
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