痰液诱导法在支气管哮喘患儿痰液细胞分析中的应用

目的 :探讨痰液诱导法在支气管哮喘 (哮喘 )患儿痰液细胞分析中的应用。方法 :6 0例哮喘患儿分为急性发作期组 (34例 )和哮喘缓解期组 (2 6例 ) ,2 5例健康儿童作为对照组。高渗盐水诱导儿童吐痰 ,瑞氏染色法进行痰液总细胞及细胞分类计数。结果 :3组最大呼气流量 (PEF)下降差异无显著性。哮喘急性发作期组痰液细胞总数(TCC)、嗜酸性粒细胞数 (E0 )、中性粒细胞数 (N)均显著高于哮喘缓解期组和正常对照组。结论 :痰液诱导法简便、安全、准确 ,通过痰液诱导分析哮喘患儿痰液细胞有临床实用价值。

Application of sputum induction in the analysis of sputum cells in children with bronchial asthma

Aim：To examine the application of the sputum induction in the analysis of sputum cells of children with bronchial asthma．Methods： Sixty children with asthma were divided into 2 groups:acute exacerbation group(34) and remission group(26) with 25 healthy children as control group.The sputum were collected by inhaling hypersonic saline．After the Wright′stain the total cell count and inflammatory cells were made．Results： The decline of PEF between 3 groups had no significant differences．The levels of the total cell count（TCC），eosinophil（E0），neutrophil（N）in the group of children with acute exacerbation of asthma were significantly higher than those in the group at recovery stage of asthma and control group． Conclusion：The sputum induction is convenient，safe and accurate．There is a clinical significance in analysing the sputum cells of children with asthma by the sputum induction．