| 小鼠牙本质涎磷蛋白5′端非编码区启动子报告基因载体的构建和分析 |
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| 引用本文： | 高杰,吴补领,何文喜,李萍,郭婷,李霞.小鼠牙本质涎磷蛋白5′端非编码区启动子报告基因载体的构建和分析[J].牙体牙髓牙周病学杂志,2005,15(2):72-75. |
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| 作者姓名： | 高杰 吴补领 何文喜 李萍 郭婷 李霞 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目 (30200315 )，全军“十五”重点课题 |
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| 摘 要： | 目的：克隆小鼠牙本质涎磷蛋白(dentin sialophosphopmtein，DSPP)基因启动子，构建含DSPP启动子不同片段的报告基因载体，在小鼠成牙本质细胞系MDPC-23中分析各种载体中DSPP启动子活性：方法：PCR、报告基因载体构建、瞬时转染和报告基因检测。结果：PCR获得DSPP启动子的3个不同片段，将它们克隆到萤火虫荧光素酶报告基因载体pG13-Enhancer，构建出3种含DSPP启动子不同片段的报告基因载体，将这些报告基因载体瞬时转染至MDPC-23细胞，载体中的启动子具有不同的活性。结论：成功构建了含小鼠DSPP启动子片段的报告基因载体，为以后研究DSPP基因表达调控的分子机制提供了实验工具。
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| 关 键 词： | 牙本质涎磷蛋白 启动子 报告基因 |
| 文章编号： | 1005-2593(2005)02-0072-04 |
| 修稿时间： | 2004年9月7日 |
Construction and analysis of mouse dentin sialophosphoprotein promoter reporter gene plasmids |
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| GAO Jie,WU Bu-ling,HE Wen-xi,et al.Construction and analysis of mouse dentin sialophosphoprotein promoter reporter gene plasmids[J].Chinese Journal of Conservative Dentistry,2005,15(2):72-75. |
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| Authors: | GAO Jie WU Bu-ling HE Wen-xi |
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| Abstract: | AIM: To clone the promoter of mouse dentin sialophosphoprotein (DSPP) gene and construct luciferase reporter gene vectors containing different DSPP promoter fragments,and to analyze the promoter activity of various constructions using mouse odontoblast cell line MDPC-23 cells. METHODS: PCR,construct luciferase reporter gene vectors,transient transfection and luciferase assay were used. RESULTS: Three different fragments of DSPP promoter were got.Then they were cloned into pGL3-Enhancer luciferase expression vector.These three DSPP promoter luciferase reporter gene vectors had different luciferase activity after transfection into MDPC-23 cells respectively. CONCLUSION: Mouse DSPP promoter luciferase reporter gene plasmids were successfully constructed,which will provide a useful tool for study of the molecular mechanism of DSPP expression in future. |
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| Keywords: | dentin sialophosphoprotein promoter reporter gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
