硒及维生素E对大鼠N_2O_4中毒性肺损伤的保护作用

目的观察补充硒(Se)与维生素E(VitE)对四氧化二氮(N2O4)致大鼠中毒性肺损伤的保护作用。方法40只Wistar大鼠被随机分成对照组、N2O4中毒组(N2O4组)、N2O4中毒+VitE组(VitE组)、N2O4中毒+Se组(Se组)、N2O4中毒+VitE+Se联合应用组(VitE+Se组)5组。使大鼠吸入N2O4(浓度352±21mg/m3,时间0.5h)复制出中毒性肺损伤模型。吸入N2O4前14dVitE组大鼠每日按200mg/(kg·bw)灌胃补充VitE;同时Se组大鼠每日按0.2mg/(kg·bw)灌胃补充Se而VitE+Se组大鼠每日按VitE200mg/(kg·bw)及Se0.2mg/(kg·bw)联合补充。中毒后3h处死大鼠,测量肺系数、血浆丙二醛(MDA)、血浆谷胱甘肽过氧化物酶(GSH-Px)、红细胞超氧化物歧化酶(SOD)以及血浆和肺内心钠素(ANP)水平。结果N2O4组大鼠的肺系数、血浆MDA及血浆ANP显著升高,血浆GSH-Px和红细胞SOD及肺内ANP明显降低。而补充VitE、Se大鼠上述指标的改变较中毒组减轻,在VitE+Se组尤其明显。结论补充VitE与Se对N2O4致大鼠中毒性肺损伤显示出有效保护作用,尤以两者联合补充效果更佳。

The protective effects of providing rats with Vitamin E and Selenium on toxic pulmonary injury induced by dinitrogen tetroxide

Objective To observe the protective effects of providing rats with Vitamin E(Vit E)and Selenium(Se)on toxic pulmonary injury induced by dinitrogen tetroxide(N 2O4 ).Methods Forty Wistar rats were randomly divided into5groups:control,N 2O4 intoxication(N 2O4 group),N 2O4 intoxication plus Vit E supplied(Vit E group),N 2O4 intoxication plus Se offered(Se group),N 2O4 intoxication plus Vit E and Se provided(Vit E+Se group).The rat pulmonary injury was induced by inhalation of N 2O4 (352±21mg/m 3 )for0.5h.For Vit E,Se and Vit E+Se groups,Vit E(200mg/kg of body weight,i.e(kg·bw)),Se(0.2mg/kg·bw)and Vit E plus Se(200mg/(kg·bw)+0.2mg/(kg·bw))were correspondingly given to rats with intragastric method for14days before inhalation of N 2O4 .The rats were killed later3h after intoxi-cation,and the parameters of lung coefficient,plasma malondialdehyde(MDA),plasma glutathione peroxides(GSH-Px),superoxide dismutase(SOD)activities of RBC,plasma and lung atrial natriuretic polypeptide(ANP)were measured.Results The lung coefficient,plasma MDA and ANP were higher while the plasma GSH-Px,SOD activities of RBC and lung ANP were lower significantly in rats of N 2O4 group when comparing with those of control.In rats of Vit E,Se and Vit E+Se groups,changes of above indexes were marked less prominent in comparison with those of N 2O4 group,and it was shown obviously in the Vit E+Se group.Conclusion Providing with Vit E and Se has effectively protective effect on rats pulmonary injury induced by N 2O4 ,and the better effect is shown in combined furnishing Vit E with Se.