HBsAg阳性慢性肝病患者肝组织HBV DNA分子状态与血清HBV标志物关系的研究

采用分子杂交技术对28例慢性肝病患者进行肝组织和血清HBV对比研究,结果表明:①肝组织HBV DNA阳性率显著高于血清HBV DNA阳性率,②血清DNA-P反映乙肝病毒的早期复制;③血清HBeAg阴性、Anti-HBe阳性时肝组织内可发现游离型HBV DNA及小片段DNA,表明肝内HBV正处于活跃复制状态,从分子水平证实了Anti-HBe阳性时部分患者可有传染性,需积极有效治疗:④慢迁肝和慢活肝中发现整合型HBV DNA较少,提示慢活肝经治疗后有希望达到从肝内清除乙肝病毒的目的。另外,也提示乙肝病毒在肝内的复制状态与病理损伤无直接关系。

Study on the Relation between Hepatitis B Virus DNA Molecularity in Hepatic Tissue and Hepatitis B Virus-Markers in.Serum of the Patients with HBsAg-positive Chronic Liver Disease

The Hepatitis B Virus DNA in hepatic tissue of 28 patients with chronic liver disease were detected by adopting the molecular hybridization technique and were contrasted with serological examination. The results showed that: 1. the HBV DNA positive rate in hepatic tissue was remarkable higher than it in serum, 2. DNA-P in serum can reflect the early replication of Hepatitis B Virus, 3. When HBeAg-negative and anti-HBe-positive in serum, the free HBV DNA and DNA frogment can still be found in hepatic tissue, it maked clear that HBV was replicating actively and confirmed at the molecular level that some of patients with anti-HBe-positive have infectivity must be treated,4. integral HBV DNA is less in patients with CPH and CAH, it points to the aim of eliminating HBV from liver will be achieved after treatment. In addition, it also points out the replicating state of HBV in liver has not direct relation with hepatic pathological picture