消幻汤含药血清对人神经母细胞瘤细胞增殖及形态的影响

目的初步探讨消幻汤含药血清对人神经母细胞瘤细胞SH—SYSY的增殖及形态的影响。方法通过连续7d给家兔灌胃给药，制备消幻汤含药兔血清、利培酮含药免血清及空白兔血清。将上述血清作用于SH—sY5Y细胞24～72h，通过倒置显微镜观察舍药血清干预48h的SH—SY5Y细胞的形态，并且每24小时采用MTT法检测SH～SY5Y细胞的增殖情况。结果消幻汤含药血清处理48h的SH—SY5Y细胞突起较长，细胞间接触紧密，并且伸出伪足，虽表现为增殖旺盛，但消幻汤含药血清对SH—SY5Y细胞的增殖与空白血清相比，差异无统计学意义（P〉0．05），而利培酮含药血清则可显著增加sH—SY5Y的细胞数量，呈现对数增殖趋势，与其他各组比较，差异有统计学意义（P〈0．05）。结论消幻汤含药血清对SH—SY5Y细胞的正常形态有促进作用，而对SH—SY5Y细胞的正常增殖无显著干预作用，与利培酮舍药血清作用不同。

Effect of Xiao Huan Tang containing serum on proliferation and morphology of human neuroblastoma

Objective To investigate the effect of Xiao Huan Tang containing serum on prolifera- tion and morphology of human neuroblastoma SH--SY5Y cells. Methods Seven consecutive days ga- vaged Xiao Huan Tang or risperidone drug--containing rabbit serum. The morphology of SH--SYSY cells was observed by inverted microscope after 48 hours drug--containing serum treated, and every 24 h proliferation of SH--SY5Y cell was tested by the MTT assay. Results Xiao Huan Tang containing ser- um treated 48 h SH--SY5Y cell were close contact between the cells, had pseudopodia, and promoted proliferation. Xiao Huan Tang containing serum compared with the blank serum on proliferation of SH-- SY5Y cells was not statistically significant （P ~ 0.05）, but risperidone containing serum got a significant increase in the number of SH--SY5Y cells, showing the logarithmic growth trend （P 〈 0.05）. Conclu- sions Xiao Huan Tang containing serum can promote the normal form of the SH--SY5Y cells, but have not significant intervention on normal proliferation of SH--SYSY cells, plays a different role from risper- idone.