消幻汤含药血清对人神经母细胞瘤细胞增殖及形态的影响 |
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引用本文: | 张燕,冀翠玲,徐堂辉,刘飞虎,师建国. 消幻汤含药血清对人神经母细胞瘤细胞增殖及形态的影响[J]. 神经疾病与精神卫生, 2013, 13(4): 335-338 |
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作者姓名: | 张燕 冀翠玲 徐堂辉 刘飞虎 师建国 |
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作者单位: | 1. 710061,西安市精神卫生中心 2. 陕西中医学院 |
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基金项目: | 国家自然科学基金项目,陕西省科学技术研究发展计划项目 |
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摘 要: | 目的初步探讨消幻汤含药血清对人神经母细胞瘤细胞SH—SYSY的增殖及形态的影响。方法通过连续7d给家兔灌胃给药,制备消幻汤含药兔血清、利培酮含药免血清及空白兔血清。将上述血清作用于SH—sY5Y细胞24~72h,通过倒置显微镜观察舍药血清干预48h的SH—SY5Y细胞的形态,并且每24小时采用MTT法检测SH~SY5Y细胞的增殖情况。结果消幻汤含药血清处理48h的SH—SY5Y细胞突起较长,细胞间接触紧密,并且伸出伪足,虽表现为增殖旺盛,但消幻汤含药血清对SH—SY5Y细胞的增殖与空白血清相比,差异无统计学意义(P〉0.05),而利培酮含药血清则可显著增加sH—SY5Y的细胞数量,呈现对数增殖趋势,与其他各组比较,差异有统计学意义(P〈0.05)。结论消幻汤含药血清对SH—SY5Y细胞的正常形态有促进作用,而对SH—SY5Y细胞的正常增殖无显著干预作用,与利培酮舍药血清作用不同。
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关 键 词: | 神经母细胞瘤 增殖 形态 消幻汤 |
Effect of Xiao Huan Tang containing serum on proliferation and morphology of human neuroblastoma |
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Affiliation: | ZHANG Yan,JI Cui-ling,XUTang-hui,etal. Xi'anMental Health Center, Xi'an 710061,China |
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Abstract: | Objective To investigate the effect of Xiao Huan Tang containing serum on prolifera- tion and morphology of human neuroblastoma SH--SY5Y cells. Methods Seven consecutive days ga- vaged Xiao Huan Tang or risperidone drug--containing rabbit serum. The morphology of SH--SYSY cells was observed by inverted microscope after 48 hours drug--containing serum treated, and every 24 h proliferation of SH--SY5Y cell was tested by the MTT assay. Results Xiao Huan Tang containing ser- um treated 48 h SH--SY5Y cell were close contact between the cells, had pseudopodia, and promoted proliferation. Xiao Huan Tang containing serum compared with the blank serum on proliferation of SH-- SY5Y cells was not statistically significant (P ~ 0.05), but risperidone containing serum got a significant increase in the number of SH--SY5Y cells, showing the logarithmic growth trend (P 〈 0.05). Conclu- sions Xiao Huan Tang containing serum can promote the normal form of the SH--SY5Y cells, but have not significant intervention on normal proliferation of SH--SYSY cells, plays a different role from risper- idone. |
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Keywords: | Neuroblastoma Proliferation Morphology Xiao Huan Tang |
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