脑星形细胞瘤p16基因缺失及5'CpG岛甲基化检测

目的探讨p16基因异常与脑星形细胞瘤发生、发展的关系。方法利用PCR及PCR-based甲基化技术检测了56例不同级别的脑星形细胞瘤组织p16基因缺失及5'CpG岛甲基化状况。结果18例高病理级别的脑星形细胞瘤发生了p16基因缺失，而低病理级别的脑星形细胞瘤无一例发生缺失（P<0.05）；6例脑星形细胞瘤发生了p16基因5'CpG岛甲基化。结论p16基因失活可能参与脑星形细胞瘤的病理发生、恶性进展。p16基因纯合缺失是p16基因失活的主要机制。

Deletion and 5'CpG island methylation of p16 gene in brain astrocytoma

Aim:To investigate if the abnormality of p16 gene is involved in the occurrence or malignant progression of brain glioma.Methods: Deletion and 5'CpG island methylation of p16 gene were detected in 56 cases with brain gliomas by the methods of PCR and PCR based methylation.Results: Homozygous deletions of p16 gene were found in 18 cases of malignant brain glioma, while deletion of p16 gene was not found in any low grade gliomas. Six cases of brain glioma were found to be methylated in 5'CpG island of p16 gene.Conclusion: Inactivation of p16 gene may be involved in occurrence or malignant progression of brain glioma. Homozygous deletion p16 of gene is the major mechanism for inactivation of p16 gene.