蚯蚓提取物体内和体外抗肿瘤作用的研究

目的探讨蚯蚓提取物对Eca-109、Hella、K562细胞的生长抑制作用及机制。方法应用四甲基偶氮唑盐(MTT)比色分析,流式细胞仪测定细胞凋亡、细胞周期及观察小鼠体内肿瘤体积和重量。结果蚯蚓提取物Ⅰ,Ⅱ,Ⅲ中只有成分Ⅱ的高、中剂量(900,450 mg/L)对Eca-109、Hella、K562细胞有明显的抑制作用。体内300,600 mg/L灌胃小鼠对肿瘤有明显的抑制作用。Hella细胞检测有凋亡细胞出现,细胞周期检测,细胞被阻滞在G0-G1期,DNA合成受阻故使肿瘤细胞受抑制,肿瘤体积缩小。结论蚯蚓提取物Ⅱ对肿瘤细胞增殖有明显的抑制作用,主要通过细胞凋亡和肿瘤细胞受阻于G0-G1期,使DNA合成减少。

Study on the anti-tumor effects of earthworm extracts both in vivo and in vitro

PurposeTo study the inhibitive effects of earthworm extract on the cell growth of Eca-109,Hella and K_(562),and its mechanisms.MethodsMTT colorimetric analysis and flow cytometry were used to determine cell apoptosis and activity period,and to observe the size and weight of the tumor in mice.ResultsAmong the Ⅰ,Ⅱ,and Ⅲ components of earth-worm extracts,only component Ⅱ of high and medial dosage(900,450 mg/L)had prominent inhibitive effects on Eca-109,Hella and K_(562) cells.And component Ⅱ(300,600(mg/L))in vivo was found to have significant inhibitive effects on tumor growth.It showed that there was appearance of Hella cell apoptosis and cells were blocked at G0-G1 stage.When DNA synthesis was blocked,the cancer cells were inhibited.Then the tumor size became smaller.ConclusionThe component Ⅱ of earthworm extracts can significantly inhibit proliferation of tumor cells through the reduction of DNA synthesis.