Alternative splicing of the FMR1 gene in human fetal brain neurons |
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Authors: | Tao Huang Lan-Ying Li Yan Shen Xue-bin Qin Zhi-Ling Pang Guan-Yun Wu |
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Institution: | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing |
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Abstract: | The alternative splicing expression of the FMR1 gene was reported in several human and mouse tissues. Five regions of FMR1 gene can be alternatively spliced, but the combination of them has not been investigated fully. We reported here the analysis of alternative splicing pattern of the FMR1 gene in cultured fetal human neurons, using a RT-PCR and cloning strategy. Eleven splicing types were cloned and different isoforms were not equally represented. The dominant isoform represents nearly 40%, and the other isoforms were relatively rare. One isoform has a different carboxyl-terminus. Most of the alternative spliced regions appear hydrophilic; thus, they may locate on the surface of the FMR1 protein. © 1996 Wiley-Liss, Inc. |
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Keywords: | FMR1 gene alternative splicing neuron development |
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