Buffering characteristics of eel blood at the extreme conditions in the swimbladder |
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Institution: | 1. Department of Neurology, University of Würzburg, Würzburg, Germany;2. Institute of Anatomy and Cell Biology II, University of Würzburg, Würzburg, Germany;1. Stem Cell and Regenerative Medicine Group, Institute of Anatomy and Cell Biology, Julius-Maximilians-Universität Würzburg, Koellikerstrasse 6, D-97070 Würzburg, Germany;2. Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, Universität Bonn Life and Brain Center and Hertie Foundation, Sigmund-Freud Strasse 25, 53105 Bonn, Germany;3. Genomics, Stem Cell Biology & Regenerative Medicine Dpt., Institute of Molecular Biology, Leopold-Franzens-University Innsbruck, Technikerstraße 25, 6020 Innsbruck, Austria |
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Abstract: | CO2 binding in whole blood and true plasma of the eel was estimated by measuring CO2 content and pH in blood aliquots equilibrated with various PCO2 values over a wide range expected to occur in the swimbladder. Bicarbonate concentration, HCO3−], was calculated using the CO2 solubility coefficient, which was measured to average 50 μmol·L−1·Torr−1 (20°C). Buffer lines of non-bicarbonate buffers were obtained in plots of HCPO3−] against pH, and non-bicarbonate buffer values, βNB, were obtained by curve. fitting.In the pH range 6.6–8.2, the buffer line for oxygenated whole blood was sigmoid, while that for deoxygenated blood increased its slope monotonously with increasing pH. The βNB for oxygenated blood displayed a maximum of about 8.6 mmol·L−1pH−1 at pH = 7.4 and dropped down to below 1 mmol·L−1·pH−1 at higher and lower pH. Similar shapes of the buffer lines were obtaned in true plasma; the HCO3−] levels and βNB values were, however, somewhat higher than in whole blood.These data are useful fo assess the back-diffusion of CO2 and HCO3− in the rete mirabile of the fish swimbladder and to estimate the effects CO2 back-diffusion exerts on the counter-current enhancement of O2 in the rete. |
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