| Foxp3基因及调节性T细胞在重症肌无力被动转移幼鼠发病中的作用机制 |
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| 引用本文： | 魏秀丽,黄志,李欣,刘玮.Foxp3基因及调节性T细胞在重症肌无力被动转移幼鼠发病中的作用机制[J].第三军医大学学报,2010,32(4). |
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| 作者姓名： | 魏秀丽 黄志 李欣 刘玮 |
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| 作者单位： | 1. 重庆医科大学附属儿童医院,神经内科,重庆,400014
2. 重庆医科大学附属儿童医院,儿科研究所,重庆,400014 |
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| 基金项目： | 重庆市卫生局科研基金(2008-2-169)~~ |
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| 摘 要： | 目的探讨Foxp3+CD4+CD25+调节性T细胞、Foxp3mRNA在重症肌无力被动转移模型(passive transferred myasthenia gravis,PTMG)发病中的作用机制。方法将16只幼年雌性C57BL/6小鼠随机分为2组:模型组和对照组,模型组经腹腔注射含1.0mg/kgmAb35的Ringer's液0.2ml建立PTMG模型,正常对照组注射不含mAb35的Ringer's液0.2ml。采用流式细胞技术检测小鼠脾细胞中CD4+CD25+T细胞及Foxp3+CD4+CD25+Tregs含量,实时荧光定量聚合酶链反应(RT-FQ-PCR)分析2组小鼠脾细胞中Foxp3mRNA的表达,ELISA法检测2组小鼠血清白介素-2和干扰素-γ的水平。结果①模型组小鼠脾细胞中CD4+CD25+T细胞与CD4+T细胞含量的比例与对照组比较有统计学差异(8.82±0.74)%vs(9.89±0.88)%,P<0.05];模型组Foxp3+CD4+CD25+Tregs与CD4+CD25+T细胞含量的比例与对照组比较显著降低(6.83±1.18)%vs(8.38±0.76)%,P<0.01];脾细胞...
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| 关 键 词： | 重症肌无力 调节性T细胞 Foxp3 IL-2 荧光定量聚合酶链反应 小鼠 近交CSTBL |
Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice |
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| Wei Xiuli,Huang Zhi,Li Xin,Liu Wei.Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(4). |
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| Authors: | Wei Xiuli Huang Zhi Li Xin Liu Wei |
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| Institution: | 1 Department of Neurology;Children's Hospital;2Hnstitute of Pediatrics;Chongqing Medical University;Chongqing;400014;china |
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| Abstract: | Objective To study the role of Foxp3 + CD4 + CD25 + regulatory T cells(Foxp3 + CD4 + CD25 + Tregs) and Foxp3mRNA in pathogenesis of passive transferred myasthenia gravis(PTMG) by detecting their expression in a PTMG model.Methods Sixteen C57BL/6 young female mice were divided into model group and control group.A PTMG model was established by injecting Ringer's solution containing 1.0 mg/kg mAb35 into abdominal cavity.Mice in control group were injected with Ringer's solution not containing mAb35.Levels of C... |
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| Keywords: | myasthenia gravis regulatory T cell Foxp3 interleukin-2 real-time fluorescent quantitative polymerase chain reaction mice inbred C57BL |
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