丙型肝炎病毒母婴垂直传播的研究

目的了解丙型肝炎病毒(HCV)母婴垂直传播情况.方法采用酶联免疫法(ELISA)和聚合酶链反应(PCR)检测1047例肝病孕产妇血清丙型肝炎病毒抗体(抗～HCV)和丙型肝炎病毒基因(HCV～RNA),对HCV感染产妇血清进行HCV分型及半定量分析.再对HCV感染产妇的丈夫静脉血及新生儿脐血、出生后24小时内、1、6、12个月股静脉血进行抗-HCV、HCV-RNA和HCV分型的检测.结果1047例肝病孕产妇中有12例(1.15%)HCV感染,其丈夫血清抗-HCV、HCVRNA均为阴性,而12名新生儿中有3例脐血及出生后24小时内外周血均抗-HCV阳性,其中2例HCV-RNA同时阳性,仅1例婴儿在出生后1、6、12个月时检测抗-HCV与HCV-RNA均阳性,且与其母所感染的HCV基因型相同,其余2例婴儿出生后1个月均阴转.本组HCV母婴传播率为8.3%.结论支持我国存在HCV母婴垂直传播.

The Study on Mother to Child Transmission of Hepatitis C Virus

Objective:To investigate the complexion of Mother to child transmission of hepatitis virus C(HCV) . Methods:Anti HCV and HCV RNA were detected by enzyme linked immunosorbent assay(ELISA) and Polymerase Chain Reaction(PCR) in 1 047 mothers with HCV or HCV RNA positive .The serum of pregnants with HCV infective were done by genotype of HCV and half quantitative analysis sa well as their husbands. Then blood samples of the newborns were collected and received at 0, 1, 6, 12 month after birth respectively and the test of the Anti HCV and HCV RNA and the genotype of HCV were determined. Results:The infection rate of HCV was 1.15% (12 persons) in 1 047 pregnant. But the serum of the anti HCV and HCV RNA were negative in their husbands. The anti HCV positive were found in the 1 samples of the newborns blood, the serum of the anti HCV and HCV RNA were positive in 2 newborns between 24 hours after birth in 12 newborns. Only one was continuance positive at 1, 6, 12 month after birth and the genotype of infection of HCV was the same as his mother. The negative were found in 2 newborns in 30 days. The rate of vertical transmission was 8.3% in newborns from HCV RNA positive mothers. Conclusions:The facts was proved in the study that the vertical transmission of hepatitis virus C was found in newborns from HCV RNA or anti HCV positive mothers in China.