参芪扶正注射液调节环磷酰胺化疗后小鼠腹腔巨噬细胞功能的研究

目的考察参芪扶正注射液对环磷酰胺化疗小鼠巨噬细胞吞噬功能和杀伤肿瘤细胞活性的影响。方法将小鼠随机分为对照组、环磷酰胺组以及参芪扶正注射液低、中、高剂量(10、20、40 m L/kg)组。除对照组外,其余各组小鼠均ip环磷酰胺100 mg/kg。参芪扶正注射液组小鼠ig给予10、20、40 m L/kg参芪扶正注射液,对照组和环磷酰胺组ig给予等量生理盐水,1次/d,连续5 d。称定胸腺和脾脏质量,计算免疫器官指数;ELISA法检测小鼠血清IL-2水平;流式细胞仪检测巨噬细胞吞噬荧光微球的数量和比例,计算吞噬率和吞噬指数;流式细胞仪检测CFSE标记的H22细胞中PI阳性细胞的比例。结果参芪扶正注射液可以不同程度地恢复环磷酰胺化疗小鼠的胸腺指数和脾指数;显著提高环磷酰胺化疗小鼠血清IL-2水平;减少小鼠巨噬细胞的吞噬率和吞噬指数;不同程度地提高环磷酰胺化疗后小鼠巨噬细胞对H22细胞的杀伤活性。结论参芪扶正注射液可改善环磷酰胺化疗后小鼠免疫抑制状态,对巨噬细胞吞噬和杀伤活性均有调节作用。

Modulation effect of Shenqi Fuzheng Injection on functions of peritoneal macrophages in cyclophosphamide treated mice

Objective To investigate the modulation effect of Shenqi Fuzheng Injection (SFI) on peritoneal macrophage phagocytosis and tumor-killing activity in cyclophosphamide (Cy) treated mice. Methods NIH mice were randomly divided into control group, Cy group, and low-, mid-, and high-dose (10, 20, and 40 mL/kg) SFI groups. Mice were ip administered with Cy (100 mg/kg) except control group. Mice in SFI groups were ig administrated with 10, 20, and 40 mL/kg SFI, once daily for 5 d, while those in control group and Cy group were ig administered with the same dosages of saline solutions. The weights of spleen and thymus were weighed to calculate the immune organ index. Levels of serum IL- 2 were determined by ELISA method. The amounts and proportions of macrophage phagocytosis of fluorescent microspheres were detected by Flow cytometry to calculate phagocytic rates and phagocytosis index. The proportion of PI positive cells in liver cancer cell H22 marked with CFSE was detected by Flow cytometry. Results SFI could recovery spleen and thymus index of Cy-treated mice in different extent, significantly improve the level of serum IL-2, and reduce phagocytic rates and phagocytosis index. SFI could also enhance the tumor-killing activity for liver cancer cell H22 of macrophages in Cy-treated mice in different degree. Conclusion SFI can improve immunosuppression, and regulate the phagocytosis function and enhance the tumor-killing activity of macrophages in Cy-treated mice.