阻断白三烯B4/白三烯B4受体1途径在川崎病治疗中的作用 |
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引用本文: | 王丹丹,邓淑珍,尹薇,孙东明,方建. 阻断白三烯B4/白三烯B4受体1途径在川崎病治疗中的作用[J]. 海南医学, 2010, 21(20): 10-11 |
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作者姓名: | 王丹丹 邓淑珍 尹薇 孙东明 方建 |
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作者单位: | 武汉市儿童医院心内科,湖北,武汉,430016 |
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摘 要: | 目的观察白三烯B4(LTB4,leukotriene B4)在川崎病患者血清中的表达及阻断白三烯B4/白三烯B4受体1(LTB4-BLT1)途径对内皮细胞游走的影响,以探讨阻断LTB4-BLT1途径在川崎病治疗中的意义。方法用酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)检测川崎病急性期及丙种球蛋白治疗后患儿的LTB4血清含量;用改良Boyden法检测加入BLT1拮抗剂U-75302后的血清对内皮细胞游走的影响。结果在川崎病急性期,血清LTB4含量明显增高,用丙种球蛋白治疗后下降;在加入BLT1拮抗剂后使得上述血清对内皮细胞的游走作用明显降低。结论 LTB4-BLT1途径参与川崎病血管炎性损伤过程,阻断LTB4-BLT1途径可能成为治疗川崎病血管内皮损伤的新靶点。
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关 键 词: | 川崎病 白三烯B4 LTB4-BLT1途径 |
Blocking the LTB4-BLT1 pathway in the curing Kawasaki disease |
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Affiliation: | WANG Dan-dan,DENG Shu-zhen,YIN Wei,et al.Department of Cardiology,Wuhan Children's Hospital,Wuhan 430016,Hubei,CHINA |
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Abstract: | Objective To investigate the effect of leukotriene B4 in the patients with Kawasaki disease.Meanwhile,the effects of human umbilical vein cell migration by blocking the LTB4-BLT1 pathway were observed.Methods The concentration of LTB4 in the serum from patients with Kawasaki disease was observed in a cure stage before and after IVIG.The migration of endothelial cells in plasma with U-75302 was determined by modified Boyden chamber methods.Results The level of LTB4 in Kawasaki disease was significantly higher than that in normal controls.Migration index showed decrease after blocking the LTB4-BLT1 pathway.Conclusion It is thought that LTB4-BLT1 pathway is involved in vascular damage in Kawasaki disease,blocking the LTB4-BLT1 pathway may be a new way to cure Kawasaki disease. |
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Keywords: | Kawasaki disease Leukotriene B4 LTB4-BLT1 pathway |
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