Quantitative assessment of salivary oral bacteria according to the severity of dental caries in childhood |
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| Institution: | 1. São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Pediatric Dentistry and Public Health, Araçatuba, SP, Brazil;2. The Forsyth Institute, Department of Microbiology, Cambridge, MA, USA;3. Harvard School of Dental Medicine, Harvard University, Boston, MA, USA;4. São Paulo State University (UNESP), School of Dentistry, Araraquara, Department of Dental Materials and Prosthodontics, Araraquara, SP, Brazil;1. Ministry of Health, Saudi Arabia;2. Department of Pediatric Dentistry, Riyadh Elm University, Riyadh, Saudi Arabia;3. Department of Microbiology and Immunology, Riyadh Elm University, Riyadh, Saudi Arabia;1. Graduate Program in Dental Science, Araçatuba Dental School, UNESP – Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil;2. Multicenter Graduate Program in Physiological Sciences, Araçatuba Dental School, UNESP – Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil;3. Department of Pediatric Dentistry and Public Heatlh, Araçatuba Dental School, UNESP – Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil;4. Department of Basic Sciences, Araçatuba Dental School, UNESP – Univ Estadual Paulista, Rua José Bonifácio, 1193, Vila Mendonça, 16015-050 Araçatuba, São Paulo, Brazil;1. Irmandade Santa Casa de Misericórdia de Porto Alegre, Rio Grande do Sul, Brazil;2. Department of Radiology, Federal University of Rio de Janeiro, Río de Janeiro, Brazil;1. Servicio de Neumología, Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain;2. Unidad de Epidemiología Clínica, Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain;3. Grupo Epidemiología de Enfermedades Frecuentes, Instituto de Investigaciones Sanitarias de Santiago (IDIS), Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain;4. Servicio de Neumología, Grupo Interdisciplinar de Investigación en Neumología, Instituto de Investigaciones Sanitarias de Santiago (IDIS), Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain |
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| Abstract: | This study aimed to assess differences in selected oral bacteria in children according to the severity of dental caries. One hundred and thirty-six children, 36–60 months old were divided into three groups according to caries status: caries-free (CF) (n = 47), early childhood caries (ECC) (n = 40) and severe-early childhood caries (S-ECC) (n = 49). Saliva was collected for detection and quantification of selected oral streptococci, Actinomyces naeslundii, Lactobacillus spp., Bifidobacterium spp., and Scardovia wiggsiae by quantitative-polymerase chain reaction. The results showed that the detection and quantitative levels of S. mutans, S. sobrinus, Bifidobacterium spp. and S. wiggsiae were significantly higher in S-ECC children compared to CF and ECC children, while for S. salivarius were significantly higher in CF compared to ECC and S-ECC children. There was no statistical difference among the clinical groups for S. mitis, S. oralis, A. naeslundii and Lactobacillus spp. levels and detection. S-ECC children had a lower monthly family income, started tooth brushing later and were breastfeed for a longer duration compared to CF children. S. mutans levels were positively correlated with S. wiggsiae and Bifidobacterium spp. levels, lower mother’s education and child bottle-feeding before sleeping and negatively correlated with S. salivarius. It was concluded that in addition to S. mutans, other bacterial species, including bifidobacteria, Scardovia wiggsiae and S. sobrinus, are associated with severity of early childhood caries, although their role in the progress of dental caries remains unclear. |
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| Keywords: | Early childhood caries Quantitative polymerase chain reaction Oral bacteria |
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