短时间吸入不同浓度七氟醚对幼鼠空间学习记忆能力及海马神经细胞凋亡的影响

目的 观察短时间吸入不同浓度七氟醚对幼鼠空间学习记忆能力及海马神经细胞凋亡的影响.方法 选择80只SD幼鼠,均为1月龄,采取随机数字表法将幼鼠分为对照组(单纯吸氧2 h)、1％七氟醚组(吸入1％七氟醚2 h)、2％七氟醚组(吸入2％七氟醚2 h)与3％七氟醚组(吸入3％七氟醚2 h),每组20只.吸入结束12 h后,各...

Influence of inhaling different concentrations of sevoflurane in a short time on spatial learning and memory ability and hippocampal neuronal apoptosis in immature rats

Objective To observe the influence of inhaling different concentrations of sevoflurane in a short time on spatial learning and memory ability and hippocampal neuronal apoptosis in immature rats.Methods Eighty SD rats aged only one month were selected and divided into control group (2 h of oxygen inhalation), 1% sevoflurane group (inhaling 1% sevoflurane for 2 h), 2% sevoflurane group (inhaling 2% sevoflurane for 2 h) and 3% sevoflurane group (inhaling 3% sevoflurane for 2 h) by random number table method, with 20 rats in each group. At 12 h after inhalation, 5 rats were selected from each group for Morris water maze test. Hippocampal tissues of 5 rats were obtained for hematoxylin-eosin (HE) staining. The neuronal apoptosis in 5 rats was determined by flow cytometry, and the concentration of free calcium ions in the neurons derived from the remaining 5 rats was detected via confocal laser scanning microscopy.Results Compared with the control group, the time spent on traveling in the quadrant where the target platform was located was shorter, and the number of times crossing the target platform was fewer in the 2% sevoflurane group and the 3% sevoflurane group (P < 0.05). The number of apoptotic neurons, as well as early apoptotic rate and late apoptotic rate of hippocampal neurons was greater in the 2% sevoflurane group and the 3% sevoflurane group relative to the control group (P < 0.05). Besides, fluorescence intensity and concentration of calcium ions in the neurons in the 2% sevoflurane group and 3% sevoflurane group were higher than those in the control group (P < 0.05).Conclusions Inhaling 2% or 3% sevoflurane rather than 1% sevoflurane in a short time will dampen the spatial learning and memory ability and promote apoptosis of hippocampal neurons, which may be triggered via the increasing level of calcium ions in the neurons.