非小细胞肺癌组织长链非编码RNA LINC00265、microRNA-98-5p的表达及其临床意义

目的 探究长链非编码RNA(lncRNA)LINC00265、microRNA-98-5p(miR-98-5p)在非小细胞肺癌(NSCLC)组织中的表达及其临床意义.方法 选取2016年1月—2017年12月乐山市人民医院收治的97例NSCLC患者的癌组织、相应癌旁正常组织进行研究.利用实时荧光定量聚合酶链反应测定癌组...

Expression and clinical significance of lncRNA LINC00265 and microRNA-98-5p in non-small cell lung cancer tissues

Objective To investigate the expression and clinical significance of long non-coding RNA (lncRNA) LINC00265 and microRNA-98-5p (miR-98-5p) in non-small cell lung cancer (NSCLC).Methods From January 2016 to December 2017, the cancer tissues and adjacent normal tissues of 97 cases of NSCLC patients admitted to our hospital were collected and studied. The expression levels of lncRNA LINC00265 and miR-98-5p in NSCLC tissues and adjacent normal tissues were determined by real-time quantitative PCR (qRT-PCR). The relationship between the expression levels of lncRNA LINC00265 and miR-98-5p and clinicopathological features and prognosis was determined. The correlation between the expression of lncRNA LINC00265 and miR-98-5p was analyzed by Pearson correlation method. The prognostic factors for NSCLC were analyzed by COX regression model.Results The expression level of lncRNA LINC00265 in NSCLC tissues was higher than that in adjacent normal tissues (P < 0.05), and the expression level of miR-98-5p in NSCLC tissues was lower than that in adjacent normal tissues (P < 0.05). The expression levels of lncRNA LINC00265 and miR-98-5p were associated with lymph node metastasis, TNM stage and degree of histologic differentiation of tumor (P < 0.05). The expression level of lncRNA LINC00265 in NSCLC tissues was negatively correlated with that of miR-98-5p (P < 0.05). The 36-month overall survival (OS) and disease-free survival (DFS) were shorter in NSCLC patients with high expression of lncRNA LINC00265 and low expression of miR-98-5p (P < 0.05). Lymph node metastasis [R = 2.152 (95% CI: 1.431, 3.235)], TNM stage [R=2.136 (95% CI: 1.429, 3.192)] and lncRNA LINC00265 [R = 2.533 (95% CI: 1.552, 4.135)] were independent risk factors for the prognosis of NSCLC patients (P < 0.05), while miR-98-5p [R = 0.618 (95% CI: 0.506, 0.755)] was the independent protective factor for the prognosis of NSCLC patients (P < 0.05).Conclusions The expression level of lncRNA LINC00265 is up-regulated and that of miR-98-5p is down-regulated in NSCLC. They may affect the occurrence and development of NSCLC synergistically, and the detection of their expression levels is helpful to determine the prognosis of NSCLC patients.