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七氟醚对结直肠癌细胞侵袭迁移的影响及对microRNA-203的调控作用机制研究
引用本文:孙飞,黄用豪.七氟醚对结直肠癌细胞侵袭迁移的影响及对microRNA-203的调控作用机制研究[J].中国现代医学杂志,2021(11):1-6.
作者姓名:孙飞  黄用豪
作者单位:天津市人民医院 麻醉科,天津 300121
摘    要:目的 观察七氟醚对结直肠癌细胞侵袭迁移的影响及对microRNA-203(miR-203)的调控作用,探讨其可能作用机制。方法 将HCT116细胞分为对照组(5%CO2培养+未转染)、七氟醚组(4%七氟醚+5%CO2培养+未转染),miR-203组(5%CO2培养+转染miR-203 mimics)、miR-203+七氟醚组(4%七氟醚+5%CO2培养+转染miR-203 mimics),miR-203-NC+七氟醚组(4%七氟醚+5% CO2培养+转染miR-203 mimics-NC)。CCK-8实验检测细胞增殖能力;qRT-PCR法检测细胞的miR-203表达;划痕实验检测细胞迁移能力;Transwell实验检测细胞侵袭能力;Western blotting法检测细胞外信号调节激酶(ERK)、p-ERK、基质金属蛋白酶-9(MMP-9)蛋白水平。结果 miR-203+七氟醚可抑制细胞增殖,降低细胞迁移能力,减少穿膜细胞数,降低细胞中p-ERK、MMP-9蛋白表达。结论 七氟醚可抑制HCT116细胞侵袭、迁移,可能是通过上调miR-203表达、阻断ERK/MMP-9信号通路发挥作用。

关 键 词:结直肠肿瘤  七氟醚  microRNA-203  HCT116细胞  ERK/MMP-9信号通路
收稿时间:2020/12/2 0:00:00

Effect of sevoflurane on invasion and migration of colorectal cancer cells based on ERK/MMP-9 signaling pathway and its regulatory effect on miR-203
Fei Sun,Yong-hao Huang.Effect of sevoflurane on invasion and migration of colorectal cancer cells based on ERK/MMP-9 signaling pathway and its regulatory effect on miR-203[J].China Journal of Modern Medicine,2021(11):1-6.
Authors:Fei Sun  Yong-hao Huang
Institution:Department of Anesthesiology, Tianjin People''s Hospital, 300121, China
Abstract:Objective To observe the effect of sevoflurane on the invasion and migration of colorectal cancer cells and the regulation of miR-203, and to explore its possible mechanism.Methods HCT116 cells were divided into control group (5% CO2 culture + untransfected), sevoflurane group (4% sevoflurane + 5% CO2 culture +untransfected), miR-203 group (5% CO2 culture + transfection with miR-203 mimics), miR-203+ sevoflurane group (4% sevoflurane + 5% CO2 culture+ transfection with miR-203 mimics), miR-203-NC+ sevoflurane group (4% sevoflurane) Ether + 5% CO2 culture+ transfection miR-203 mimics-NC). CCK-8 method was used to detect cell proliferation; qRT-PCR was used to detect the expression of miR-203 in cells; scratch test was used to detect cell migration ability; Transwell test was used to detect cell invasion ability; Western blotting method was used to detect extracellular signal-regulated kinase (ERK), p-ERK, and matrix metalloproteinase-9 (MMP-9) protein levels.Results MiR-203+ sevoflurane can inhibit cell proliferation and reduce cell migration ability, the number of penetrating cells, and the expression of p-ERK and MMP-9 proteins in cells.Conclusion Sevoflurane can inhibit the invasion and migration of HCT116 cells, possibly with up-regulating the expression of miRNA-203 and blocking the ERK/MMP-9 signaling pathway.
Keywords:colorectal neoplasms  sevoflurane  microRNA-203  HCT116 cells  the ERK/MMP-9 signaling pathway
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