Role of proteoglycans on the biochemical and biomechanical properties of dentin organic matrix |
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| Institution: | 1. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL, 60612, USA;2. Department of Operative Dentistry, College of Dentistry, University of Iowa, 801 Newton Rd, Iowa City, IA, 52242, USA;3. School of Dentistry, University of Passo Fundo, BR 285, São José,Building A7, Passo Fundo, RS, 99052-900, Brazil;1. Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;2. Division of Oral and Maxillofacial Surgery, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;3. Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;4. Midwest Biomedical Research Foundation, Research Service, Department of Veterans Affairs Medical Center, Kansas City, Missouri, USA;5. Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan;1. Department of Restorative Dentistry, College of Dentistry, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil;2. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, Illinois;3. Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil;1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;2. The State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China;3. Department of Conservative Dentistry and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China;1. Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, 1780 Hirooka-gobara, Shiojiri, Nagano 399-0781, Japan;2. Department of Oral Histology, Matsumoto Dental University, 1780 Hirooka-gobara, Shiojiri, Nagano 399-0781, Japan;1. Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Zip Code, 17012-901, Bauru, SP, Brazil;2. School of Dentistry, Centro Universitário Sagrado Coração – UNISAGRADO, Rua Irmã Arminda, 10-50, Jardim Brasil, Zip Code, 17011-160, Bauru, SP, Brazil;3. Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, Oregon Health & Science University, OHSU, 2730 SW Moody Ave, Zip Code, 97201, Portland, OR, USA;4. University Centre Integrated Faculties of Ourinhos, Rodovia BR-153, Km 338 S/N Água do Cateto, Zip Code, 19909-100, Ourinhos, SP, Brazil;5. Department of Operative Dentistry, College of Dentistry, University of Iowa, Iowa City, IA, USA, 801 Newton Rd, DSB S245, Zip Code, 52242, Iowa City, IA, USA;6. Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland and Helsinki University Hospital, Helsinki, Zip Code, 90220, Oulu, Finland;7. Research Unit of Oral Health Sciences and Medical Research Center Oulu (MRC Oulu), Oulu University Hospital, Kajaanintie 50, Zip Code, 90220, Oulu, Finland;1. School of Dentistry, University of Missouri – Kansas City, Kansas City, MO, 64108, USA;2. Department of Chemistry, University of Missouri - Kansas City, MO, 64110, USA |
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| Abstract: | ObjectiveProteoglycans (PGs) are multifunctional biomacromolecules of the extracellular matrix of collagen-based tissues. In teeth, besides a pivotal regulatory role on dentin biomineralization, PGs provide mechanical support to the mineralized tissue and compressive strength to the biosystem. This study assessed enzymatic protocols for selective PGs removal from demineralized dentin to determine the roles of these biomacromolecules in the bulk mechanical properties and biostability of type I collagen.MethodsSelective removal of glycosaminoglycans chains (GAGs) and PGs from demineralized dentin was carried out by enzymatic digestion protocols using chondroitinase ABC (c-ABC) and trypsin (Try). A comprehensive study design included assessment of dentin matrix mass loss, biodegradability of the PGs/GAGs-depleted dentin matrix, ultimate tensile strength (UTS) and energy to fracture tests. Quantitative data was statistically analyzed by two-way and one-way ANOVA followed by the appropriate post hoc tests (α = 0.05).ResultsTransmission electron microscopy images show effective GAGs removal by c-ABC and Try and both enzymatic methods released statistically similar amounts of GAGs from the demineralized dentin. Try digestion resulted in about 25% dentin matrix mass loss and increased susceptibility to collagenolytic digestion when compared to c-ABC (p = 0.0224) and control (p = 0.0901). Moreover, PGs digestion by Try decreased the tensile strengths of dentin. Statistically lower energy to fracture was observed in c-ABC-treated dentin matrix.ConclusionsGAGs plays a pivotal role on tissue mechanics and anisotropy, while the core protein of PGs have a protective role on matrix biostability. |
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| Keywords: | Dentin Proteoglycans Glycosaminoglycans Ultimate tensile strength Biodegradation |
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