腹泻型肠易激综合征患者肠黏膜PRDX1、FXR的表达及其相关性分析

目的 探讨腹泻型肠易激综合征(IBS-D)患者肠黏膜PRDX1、FXR的表达及其相关性.方法 选取2016年5月—2018年9月在海口市中医医院就诊的81例IBS-D患者为观察组,根据肠易激综合征病情严重程度量表(IBS-SSS)积分将观察组分为3个亚组,19例轻度组(75～175分),45例中度组(>175～300分...

The expressions of PRDX1 and FXR in intestinal mucosa of patients with diarrhea-predominant irritable bowel syndrome and their correlations with disease severity

Objective To investigate the expressions of peroxiredoxin 1 (PRDX1) and farnesoid X receptor (FXR) in intestinal mucosa of patients with diarrhea-predominant irritable bowel syndrome (IBS-D) and to analyze their correlations with disease severity.Methods The 81 patients with IBS-D who were treated in Haikou Hospital of Traditional Chinese Medicine from May 2016 to September 2018 were selected as the observation group. According to the Irritable Bowel Syndrome Symptom Severity Scale (IBS-SSS) score, the observation group was divided into three subgroups, with 19 cases of mild group (75 to 175 points), 45 cases of moderate group (175 to 300 points) and 17 cases of severe group (more than 300 points). Another 90 healthy and age-matched people undergoing physical examination during the same period in Haikou Hospital of Traditional Chinese Medicine were selected as the control group. HE staining was used to observe the histological morphology and inflammatory response of intestinal mucosa. The expressions of PRDX1 and FXR in the intestinal mucosa were detected by immunohistochemistry. Besides, Spearman correlation analysis was performed to observe the relationship between the expressions of PRDX1 and FXR in intestinal mucosa and IBS-SSS score.Results The positive expression rate of PRDX1 in intestinal mucosa of patients in the observation group was higher than that in the control group (P < 0.05), and the positive expression rate of FXR was lower than that of the control group (P < 0.05). The positive expression rate of PRDX1 was higher in severe group than that in mild group and moderate group (P < 0.05). Besides, the positive expression rate of FXR in the mild group was higher than that in moderate group and severe group (P < 0.05), while that in moderate group was higher compared with severe group (P < 0.05). The IBS-SSS score of mild group, moderate group and severe group increased sequentially (P < 0.05). Spearman correlation analysis showed that the positive expression rate of PRDX1 was positively correlated with IBS-SSS score (P < 0.05), and that the positive expression rate of FXR was negatively correlated with IBS-SSS score (P < 0.05).Conclusions The positive expression rate of PRDX1 is high but that of FXR is low in intestinal mucosa of IBS-D patients, yet both of them are correlated with IBS-SSS score. Thus, PRDX1 and FXR may be biomarkers and therapeutic targets of IBS-D.