Anti-inflammatory effect of an adhesive resin containing indomethacin-loaded nanocapsules |
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| Institution: | 1. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil;2. School of Dentistry, University of Federal District, Brasilia, DF, Brazil;3. Pharmacology and Therapeutic − Department of Pharmacology, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil;4. Pharmacology of Pain and Neuromodulation Laboratory, Pre-clinical Researchs, Department of Pharmacology, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil;5. Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil;6. Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil;7. Institute of Chemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil;1. Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil;2. Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil;3. Department of Periodontology and Translational Biology Research, Forsyth Institute, Cambridge, Massachusetts;4. Division of Periodontology, Department of Diagnostics and Surgical Sciences, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada;6. Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada;1. Dental Materials Laboratory, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;2. Restorative Dentistry, School of Dentistry, Federal Fluminense University, Niterói, RJ, Brazil;3. Nucleus for Dental Biomaterials Research, School of Dentistry, Veiga de Almeida University, Rio de Janeiro, RJ, Brazil;4. School of Materials Engineering, Universidade Federal de Pelotas, Pelotas, RS, Brazil;5. Organic Chemistry Department, Chemistry Institute, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;1. Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil;2. São Paulo University (USP), Al. Octávio Pinheiro Brisola, 9-75, Bauru 17012-901, Brazil;1. Departamento de Dentística, Endodontia e Materiais Dentários, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Brazil;2. Dental Materials Department, School of Dentistry, Universidade Federal do Rio Grande do Sul, Brazil |
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| Abstract: | ObjectiveTo analyze the anti-inflammatory and analgesic effects of an adhesive resin containing indomethacin-loaded nanocapsules in rat model.DesignAdhesive resin disks with or without indomethacin-loaded nanocapsules were subcutaneously implanted into right hind paw of rats. A week after surgical procedure, 2% formalin solution was intradermally injected into plantar surface of paw. Nociceptive and inflammatory responses were evaluated by formalin test. Paw edema by pletismometer and mechanical hyperalgesia by von Frey test were performed on day 2, day 4, day 6, day 8, day 10 and day 12 after surgery. IL-6, IL-10, and lactate dehydrogenase (LDH) serum levels were determined by ELISA-sandwich test.ResultsGroup containing indomethacin-loaded nanocapsules (NC) presented lower edema in the right hind paw at 24 h after formalin injection than those of the control group (CT) (P < 0.01). NC group showed decrease in the nociceptive response in phase I (neurogenic pain) compared to CT group (NC − 66.86 ± 22.83 s X CT − 130.17 ± 35.83s , P < 0.001). NC group presented supporting higher intensity of stimulus on days 8 and 12 (24 h and 72 h after formalin injection) (P < 0.01 and P < 0.02 respectively). The IL-6 serum level was also significantly higher in the NC group than CT group (p < 0.001).ConclusionsThese results indicate that an adhesive resin containing indomethacin-loaded nanocapsules has anti-inflammatory and nociceptive activities in a chemical model of acute inflammation. The present investigation confirms an adhesive resin with drug-loaded nanocapsules may be useful for improving therapeutic effect for adhesives to be used in deep cavities. |
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| Keywords: | Non-steroidal anti-inflammatory agents Indomethacin Drug carriers Dental bonding Drug release |
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