| 16S rDNA检测在临床细菌检验中的应用 |
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| 引用本文： | 詹銮峰,高鹏. 16S rDNA检测在临床细菌检验中的应用[J]. 中国实验诊断学, 2011, 15(7): 1140-1142 |
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| 作者姓名： | 詹銮峰 高鹏 |
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| 作者单位： | 1. 江西师范大学医院,江西,南昌,330027
2. 中国科学院大连化学物理研究所,辽宁,大连,116027 |
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| 摘 要： | 目的根据细菌16S rDNA的高度保守性,设计合成所有细菌的通用引物,建立快速检测细菌的方法。方法应用PCR方法检测细菌16S rDNA基因。同时对白假丝酵母菌、人白细胞DNA和HBV-DNA阳性病人血清同法检测。结果所有检测细菌均出现特异性条带,而白假丝酵母菌、人白细胞DNA和HBV-DNA阳性病人血清阴性。结论该法快速、特异、敏感。一个样品中能同时检测多种细菌,可为实验室初步判断是否存在细菌感染提供客观依据。
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| 关 键 词： | 细菌 16SrDNA 聚合酶链反应 |
Application of 16S rDNA for the detection of clinical bacteria |
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| ZHAN Luan-feng,GAO Peng. Application of 16S rDNA for the detection of clinical bacteria[J]. Chinese Journal of Laboratory Diagnosis, 2011, 15(7): 1140-1142 |
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| Authors: | ZHAN Luan-feng GAO Peng |
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| Affiliation: | 1.Jiangxi Normal University Hospital,Nachang 330027,China;2.Dalian Institute of Chemical Physics,The Chinese Academy of Sciences,Dalian 116027,China) |
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| Abstract: | Objective According to the high conservative region of 16S rDNA gene in bacteria,PCR primers of the broad-range bceteria were synthesized to establish a method to rapidly detect bacteria.Methods Bacteria 16S rDNA gene were detected by PCR method.Candida albicans and human WBC DNA and the patient serum positive for HBV-DNA were detected by the same method simultaneously.Results The detected bactiria were all presented specific band,Candida albicans and human WBC DNA and the patient serum positive for HBV-DNA were all negative.Conclusion This protocol is rapid,specific,sensitive and capable of detecting multiple organisms in a single sample.This technology is of great utility for pilot laboratory judgement on the presence of bacteria. |
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| Keywords: | 16SrDNA |
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