| 高效液相色谱法测定路边菊中绿原酸含量 |
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| 引用本文： | 梁聂彦,陈学松.高效液相色谱法测定路边菊中绿原酸含量[J].中国药业,2012,21(23):21-22. |
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| 作者姓名： | 梁聂彦 陈学松 |
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| 作者单位： | 1. 广西壮族自治区岑溪市人民医院,广西梧州,543200
2. 广西壮族自治区梧州市食品药品检验所,广西梧州,543002 |
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| 摘 要： | 目的建立测定路边菊中绿原酸含量的高效液相色谱法。方法色谱柱为AgilentSB—C18柱（250mm×4．6mm，5μm），流动相为乙腈-0．1％磷酸，检测波长为348nm，柱温为35℃。结果绿原酸进样量在0．02605．1- 6672μg之间和峰面积具有良好线性关系，线性回归方程为Y=1846039x-10829，r=0．9998（n=5）；测得10批路边菊中的绿原酸平均含量为0．19％。结论该方法简便可行，重现性好。可作为路边菊的质量控制方法。
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| 关 键 词： | 路边菊 高效液相色谱法 绿原酸 含量测定 |
Determination of Chlorogenic Acid in Boltonia Indica(L.) by HPLC |
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| Liang Nieyan
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Chen Xuesong.Determination of Chlorogenic Acid in Boltonia Indica(L.) by HPLC[J].China Pharmaceuticals,2012,21(23):21-22. |
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| Authors: | Liang Nieyan Chen Xuesong |
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| Institution: | 1.Cengxi People’s Hospital,Wuzhou,Guangxi,China 543200;2.Wuzhou Intitute for Food and Drug Control,Wuzhou,Guangxi,China 543002) |
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| Abstract: | Objective To establish the determination method of chlorogenic acid in Boltonia indica（L. ） by HPLC. Methods The column was Agilent SB-C18（250 mm ×4.6 mm, 5 μm）,mobile phase was acetonitrile and 0.1% phosphoric acid,the detection wavelength was 348 nm,column temperature was 35℃. Results Picrinine sample volume and the peak area had a good linear relationship in 0. 026 05 1. 667 2 μg,linear equation was Y = 1 846 039 X - 10 829, r = 0. 999 8 （ n = 5） ; the average content of chlorogenic acid in Boltonia indica （L.） was 0. 19%. Conclusion The method is simple,feasible and reproducible and can be used as the quality control method of Boltonia indica（L. ）. |
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| Keywords: | Boltonia indica（L ） HPLC chlorogenic acid content determination |
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