| 高效液相色谱法测定四乙酰葛根素的含量及有关物质 |
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| 引用本文： | 胡人月,张毅,陈国庆,杜洪飞,杨大坚.高效液相色谱法测定四乙酰葛根素的含量及有关物质[J].中国药业,2012,21(23):12-13. |
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| 作者姓名： | 胡人月 张毅 陈国庆 杜洪飞 杨大坚 |
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| 作者单位： | 重庆市中药研究院,重庆,400065 |
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| 基金项目： | 国家科技部重大专项项目;项目 |
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| 摘 要： | 目的建立测定四乙酰葛根素的含量及有关物质的高效液相色谱法。方法采用AgilentXDB—C18色谱柱（250mm×4．6mm，5μm），流动相为乙腈一水（45：55），流速为1．2mL／min，检测波长为250nm，柱温为30℃。结果四乙酰葛根素的质量浓度在0．1038～0．9342μg范围内与峰面积线性关系良好，Y=12871x-24．554，r=0．9999（n=7），检测限为6．65ng／mL（S／N=3）；平均加样回收率为99．94％，RSD=1．32％（n=9），有关物质含量为1．02％-1．28％。结论该方法简便、准确，可用于四乙酰葛根素的含量测定。
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| 关 键 词： | 四乙酰葛根素 高效液相色谱法 含量测定 有关物质 |
Determination of Content and Related Substances of Tetra-Acetylated Puerarin by HPLC |
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| Hu Renyue
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Zhang Yi
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Chen Guoqing
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Du Hongfei
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Yang Dajian.Determination of Content and Related Substances of Tetra-Acetylated Puerarin by HPLC[J].China Pharmaceuticals,2012,21(23):12-13. |
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| Authors: | Hu Renyue Zhang Yi Chen Guoqing Du Hongfei Yang Dajian |
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| Institution: | (Chongqing Academy of Chinese Materia Medica,Chongqing,China 400065) |
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| Abstract: | Objective To establish an HPLC method for determination of the content and related substances of tetra- acetylated puerarin （TAP）. Methods Agilent XDB- C18 chromatographic column（250 mm ×4. 6 mm,5 μm）was used and the mobile phase consisted of acetonitrile-water（45：55） with a flow rate of 1.2 mL/min. The UV detection wavelength was set at 250 nm. The column temperature was controlled at 30℃. Results The calibration curve was linear within the range of 0. 103 8- 0. 934 2μg, Y= 12 871X-24. 554, r = 0. 999 9 （ n = 7）. The detective limit was 6.65 ng/mL（ S/N = 3）. The mean recoveries was 99.94% （ RSD = 1.32% , n = 9）. The content of related substances of TAP was in the range of 1.02% -1.28%. Conclusion The method is simple,accurate and is applicable for the quality control of TAP. |
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| Keywords: | tetra - acetylated puerarin HPLC content determination related substances |
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