选育甘草乌新Ⅰ号的生理特性和基因多态性研究

目的 对从野生甘草中选育出的新品种甘草(乌新Ⅰ号)进行种质评价。方法 观察了其发芽率、可溶性蛋白量和过氧化物酶活性，并与对照组进行了基因组多态性比较。结果 乌新Ⅰ号可溶性蛋白量产生了变化，过氧化物酶活性显示乌新Ⅰ号具有较高活性。通过分子标记简单序列重复技术(ISSR)进行多态性分析，在所使用的22条随机引物中，6条随机引物产生了不同DNA条带，表明乌新Ⅰ号甘草与对照组相比产生了基因多态性变化。结论 这些变化表明乌新Ⅰ号是一个具有前景的品种并且能在将来广泛应用。

Physiological features and genetic polymorphism of a new breed licorice Wuxin No.1

Objective A new breed of licorice seeds (Wuxin No.1) was selected from wild licorice, Glycyrrhiza uralensis. Methods Its germination rate, content of soluble protein, and peroxidase (POD) activities were investigated and compared with the wild licorice seeds (control group). The leaves of Wuxin No.1 were collected and its genetic polymorphism was analyzed by inter-simple sequence repeat technique (ISSR). Results The results suggested that the seed vitality in Wuxin No.1 was higher than that in the control group. The change of soluble protein and POD activity also demonstrated that the seeds in Wuxin No.1 have the higher vigor. ISSR Analysis showed that among 22 random primers used in this experiment, six primers generated different DNA band types, which meant that there was genetic polymorphism in Wuxin No.1. Conclusion All these changes indicate that Wuxin No.1 is a prospective domestication species of licorice and may be cultivated widely in the future.