| LPS诱导肺微血管内皮细胞ICAM-1的表达及NF-κB作用的实验研究 |
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| 引用本文： | 顾大勇,曾祥元,陈莉,杨季云,马布仁,王天然,唐旭东,石力. LPS诱导肺微血管内皮细胞ICAM-1的表达及NF-κB作用的实验研究[J]. 中国微循环, 2002, 0(1) |
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| 作者姓名： | 顾大勇 曾祥元 陈莉 杨季云 马布仁 王天然 唐旭东 石力 |
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| 摘 要： | 目的研究LPS诱导的肺微血管内皮细胞 (PWVEC)ICAM -1的表达及调控机制。方法100ng/mlLPS刺激PMVEC0h、2h、4h、6h、8h或10ng/ml、50ng/ml、100ng/mlLPS刺激6h ,免疫细胞化学检测PMVECICAM -1的表达。凝胶电泳迁移率变化分析检测NF -κB的活化。并通过加入活化阻断剂PDTC观察对PMVECICAM -1表达的影响。结果PMVECICAM -1的表达与LPS的刺激呈时相 -剂量依赖方式。LPS的刺激迅速活化NF -κB ,60min达到高峰 ,后逐渐下降。PDTC能显著降低ICAM -1的表达 (P<0 .01)。结论LPS刺激诱导NF-κB的活化 ,启动ICAM -1的合成表达。
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| 关 键 词： | LPS 肺微血管内皮细胞 ICAM-1 NF-κB |
An Experimental Study on ICAM-1 Expression of Pulmonary Microvascular Endothelial Cells Induced by LPS and Its Mechanism Through Nuclear Factor-kappa B Pathway |
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| GU Da_yong,ZENG Xiang_yuan,CHEN Li,et al.. An Experimental Study on ICAM-1 Expression of Pulmonary Microvascular Endothelial Cells Induced by LPS and Its Mechanism Through Nuclear Factor-kappa B Pathway[J]. Journal of Chinese Microcirculation, 2002, 0(1) |
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| Authors: | GU Da_yong ZENG Xiang_yuan CHEN Li et al. |
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| Affiliation: | GU Da_yong,ZENG Xiang_yuan,CHEN Li,et al. Clinical Experintal Institute of Chendu Army General Hospital 610083,China |
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