急性白血病患者血清血管内皮细胞生长因子和肿瘤相关物质群水平及临床意义

目的检测急性白血病患者血清血管内皮细胞生长因子（VEGF）及肿瘤相关物质群（TSGF）的水平,探讨血清二者的水平与急性白血病的发生、发展及预后的关系。方法55例急性白血病（AL）住院患者,其中急性淋巴细胞白血病（ALL）31例,急性髓细胞白血病（AML）24例,正常人对照组15例。应用双抗体夹心酶联免疫吸附试验（ELISA）检测血清VEGF的水平,用化学显色法检测血清TSGF的水平,比较VEGF、TSGF水平在治疗前后的变化并观察VEGF、TSGF之间的相关性。结果①ALL组、AML组血清VEGF及TSGF水平均明显高于正常人对照组（P〈0.01）。②将AL患者根据第一次化疗的疗效分为完全缓解（CR）组和未完全缓解（NR）组。AML患者NR组化疗前血清VEGF及TSGF水平明显高于CR组（P〈0.05）。③AL患者血清VEGF与TSGF水平成显著正相关（r=0.5263,P〈0.01）。结论①血清VEGF及TSGF水平可作为监测急性白血病的疗效指标。②血清TSGF水平可作为急性白血病的预后判断指标,血清VEGF水平对AML的预后判断有一定意义。③AL患者血清VEGF及TSGF水平存在显著正相关。

Serum levels of vascular endothelial growth factor and tumor supplied group factors and their clinical significance in patients with acute leukemia

Objective： To determine the serum levels of VEGF and TSGF in patients with AL and to study the relationship between the levels of VEGF and TSGF and the occurrence, progression and prognosis of AL. Methods： Fifty - five patients with AL were chosen, including 31 cases of acute lymphocyte leukemia （ALL） and 24 cases of acute myelogenous leukemia（AML）. Serum levels of VEGF were measured by enzyme-linked immunosorbent assay（ELISA）. Serum levels of TSGF were measured by colorimetric estimation. The variation of VEGF and TSGF levels of AL patients were compared before and after treatment and the correlation between VEGF and TSGF were observed. Results： ①Serum VEGF and TSGF levels of ALL,AML were higher obviously than those of normal subjects and the difference was significant （P 〈 0.01 ） ; ② AL were divided into complete remission （CR） group and not complete remission （NR） depending on the reaction to the first chemical therapy. In AML patients, serum VEGF and TSGF levels of NR group before treatment were higher than those of CR group and the difference was significant （ P 〈 0. 05 ） ; ③Positive correlation was shown between serum VEGF and TSGF（ r = 0. 5263, P 〈 0. 01 ）. Conclusion ：①Serum VEGF and TSGF are useful items for detecting the effect of treatment in ALL and AML. ②Serum TSGF is a valuable item for prognosis evaluation in both ALL and AML, while significance of VEGF for evaluation prognosis is shown in AML. ③Positive correlation is shown between serum VEGF and TSGF in AL.