灵芝多糖对HepG2细胞诱导的肝癌小鼠肠道菌群及其菌群代谢功能的调节作用

目的 研究灵芝多糖对HepG2细胞诱导的肝癌小鼠肠道菌群及其菌群代谢功能的调节作用.方法 采用HepG2细胞构建肝癌小鼠模型,将21只造模成功的肝癌小鼠随机分为模型组、灵芝多糖(GLP)和益生菌处理组,每组7只,另选择正常小鼠7只作为对照组,给予药物处理组动物GLP或益生菌灌胃,另两组采用生理盐水灌胃,连续干预2 w....

Regulation of intestinal floras and their metabolism functions by ganoderma lucidum plysaceharide in mice with HepG2 cell-induced implanted cancer

Objective The aim of this experimental study was to implore the regulation of intestinal floras and their metabolism functions by ganoderma lucidum plysaceharide (GLP) in mice with HepG2 cell-induced implanted cancer. Methods The subcutaneous cancer in mice was established by HepG2 cell implantation, and 21 mice with successful implanted cancer were randomly divided into model, GLP-intervened and probiotics-intervened groups, with 7 in each. 7 normal mice were selected as control. The GLP and probiotics were administered intragastrically, while normal saline were given by gavage in model and control for 2 weeks. The diversity of intestinal floras, and fatty acid metabolites were detected by microscopy and denaturing gradient gel electrophoresis (DGGE). Results The number of Bifidobacterium, Lactobacillus, Escherichia Coli and Enterococcus in model group were(2.3±0.5)×10¹⁰ CFU/g, (12.2±2.2)×10⁹ CFU/g, (3.9±1.5)×10⁷ CFU/g and(5.0±1.2)×10¹⁰ CFU/g, all significantly higher than [(0.94±0.18)×10¹⁰ CFU/g,(3.49±0.66)×10⁹ CFU/g, (1.12±0.11)×10⁷ CFU/g and (0.57±0.06)×10¹⁰ CFU/g, respectively,P<0.05] in the control,ylajj@163.com while they were(1.1±0.1)×10¹⁰ CFU/g, (6.5±1.1)×10⁹ CFU/g, (1.8±0.2)×10⁷ CFU/g and (1.5±0.1)×10¹⁰ CFU/g in GLP-intervened group (P<0.05); the abundance, the diversity index and uniformity of intestinal floras in GLP-intervened group were (7.2±1.1)S,(4.8±1.1)H and (1.0±0.2)E, all significantly higher than [(5.7±1.2)S, (3.4±0.5)H and (0.7±0.1)E, respectively, P<0.05] in the model; the intestinal flora acetic acid, propionic acid and n-butyric acid in GLP-intervened group were (37.9±3.3)mmol/L,(3.9±0.2)mmol/L and (2.3±0.3)mmol/L, all significantly higher than [(26.8±3.2)mmol/L,(1.8±0.4)mmol/L and (1.8±0.2)mmol/L, respectively, P<0.05], while the D-lactic acid was (0.2±0.0)g/L, much lower than (0.5±0.1)g/L in the model (P<0.05). Conclusion The imbalance of intestinal floras is closely related to the carcinogenesis in mice, and the GLP administration might correct the intestinal microecological disorders, which warrants further investigation.