胆总管结扎诱导大鼠肝肺综合征模型的建立初步研究

目的 探讨胆总管结扎术诱导大鼠肝肺综合征(HPS)模型的建立.方法 40只SD大鼠被随机分为实验组30只和对照组(假手术组)10只,采用胆总管结扎术建立肝硬化和HPS模型.使用血气分析仪测定PaO2、PaCO2和pH.结果 在术后1 w,腹部超声检查显示实验组肝脏回声增粗,胆总管轻度扩张.在术后2 w,肝脏被膜不平整,...

Establishment of hepatopulmonary syndrome induced by common bile duct ligation in rats

Objective To establish a hepatopulmonary syndrome(HPS)model in rats by ligation of common bile duct.Methods 40 SD rats were randomly divided into experimental(n=30)and control group(n=10),and the rats had operation of common bile duct ligation or sham-operated.Two rats in experimental group and control group were sacrificed at 1,2,4,5,6 weeks after operation,respectively.PaO2,PaCO2 and pH were detected by blood gas analysis.Results At one week,the results of abdominal ultrasonography showed that the echo of the livers was coarse,and the bile duct was slightly dilated;at two weeks,the bile duct was obviously enlarged,and at four to six weeks,the surface of the liver was not flat in the experimental group;the histopathological examination showed that the infiltration of inflammatory cells,the formation of fibrous cords and some pseudolobules in liver tissues,and the dilatation of pulmonary microvessels in the lung tissues were observed;At the end of six week experiment,blood gas analysis showed that PaO2 was(65.5±8.2)mmHg in the experimental group,significantly lower than【(95.5±4.5)mmHg,P<0.05】in the control,serum nitric oxide level in the experimental group was(64.1±4.5)μmol/L,significantly higher than【(32.8±4.3)μmol/L,P<0.05】in the control.Conclusion The model of hepatopulmonary syndrome is successfully established by ligation of common bile duct in our experiment,which has the advantages of short model formation,high stability,and no toxicity.