非小细胞肺癌Fas/FasL基因蛋白的表达及其临床意义

目的：研究Fas/FasL基因蛋白在非小细胞肺癌（NSCLC）的表达及其临床意义。方法：应用链霉菌抗生物素蛋白－过氧化物酶（SP）法染色技术测定65例NSCLC组织标本Fas/FasL基因蛋白的表达水平，并分析其与病理类型、区域淋巴结转移、病理分级及TNM分期之间的关系。结果：NSCLC组织中Fas基因蛋白表达率显著低于正常肺组织（46．15％vs80.00%,P＜0．05），而FasL基因蛋白表达率显著高于正常肺组织（49．23%vs13.33%,P＜0．05）；FasL的表达与肺癌区域淋巴结转移有关，但Fas/FasL与肿瘤病理类型、病理分级及TNM分期无关。结论：Fas/FasL基因蛋白可能参与了非小细胞肺癌细胞凋亡的调节；检测FasL基因蛋白的表达有可能为临床判断NSCLC区域淋巴结转移、病变的发展以及评估预后提供依据。

The expression of Fas/FasL gene protein and its chinical significance in human non-small cell lung cancer

Objectives: To study the clinical significance of the expression of Fas/FasL gene protein in non small cell lung cancer(NSCLC). Methods: By using SP immunohistochemical method with anti Fas and FasL multi clonal antibody, we detected the expression of Fas/FasL gene protein in 65 cases of NSCLC and studied its correlation with histological types,local lymph node metastases, pathological grades and TNM stages. Results: The positive expression rate of Fas(46.15%) in NSCLC was much lower than that(80.00%) in the normal lung tissues( P <0.05),but the expression rate of FasL( 49.23 %) in NSCLC was much higher than that(13.33%) in the normal lung tissues( P <0.05). FasL was correlated with the stage of local lymph node metastases, but none of them had any correlation with histological types,pathological grades or the TNM stages( P >0.05). Conclusions: The data demonstrated that Fas/FasL might involve in the regulation of apoptosis of NSCLC,and the expression of FasL might be used as an objective indicator of the metastatic potency of local lymph node, extent of invation and the prognosis in NSCLC.