脂多糖诱导外周血嗜酸粒细胞释放炎性细胞因子的探讨

目的：探讨脂多糖（LPS）对嗜酸粒细胞释放炎性细胞因子白细胞介素-1β（IL-1β）、IL-2、IL-4、IL-6、IL-10、IL-12、干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）的影响。方法：嗜酸粒细胞用CD16抗体磁珠方法从人新鲜外周血中分离；分别进行5×10^5／ml嗜酸粒细胞的单独培养和与LPS（1ug／ml）共培养18h；应用流式细胞小球微阵列（cytometric bead array，CBA）方法定量测定细胞培养上清液中细胞因子含量。结果：从外周血中新分离的嗜酸粒细胞单独培养过程中没有或仅有极少量上述炎性细胞因子释放，但新分离的嗜酸性粒细胞与LPS共培养18h后，其培养上清液中IL-1β、IL-6、IL-10和TNF-α大量增加（P均〈0．001），而IL-2、IL-4、IL，12和IFN-γ的含量无明显变化。结论：LPS对嗜酸粒细胞释放炎性细胞因子具有很强的选择性诱导作用。

Study on the effects of lipopolysaccharide in inducing human eosinophils to release pro-inflammatory cytokines

Objective：To investigate the role of lipopolysaccharide （LPS） in activating human eosinophils to release inflammatory cytokines, including interleukin-1β （IL-1β）, IL-2, IL-4, IL-6, IL-10, IL-12, interferon-γ（ IFN-γ）, and tumor necrosis factor-α （TNF-α）. Methods： Eosinophils were purified from fresh human human coat using magnetic cell sorting with anti-CD16 magnetic beads. Eosinophils （5× 10^5/ml） were cultured with LPS （1ug/ml） for 18 hours. The cytokines in supernatant of eosinophil culture were determined by cytometric bead array （CBA）. Results：Eosinophils when cultured alone released little or undetectable amount of cytokines. However, when they were cultured with an addition of LPS （1 ug/ml） for 18 hours, the contents of IL-1β,IL-6 ,IL-10 and TNF-α were significantly increased in culture supernatant （all P〈0. 001）, but no significant change in content of IL-2, IL-4, IL-12 and IFN-γ. Conclusion： LPS can effectively activate eosinophils to selectively release inflammatory cytokines.