| 针刺对脑缺血胶质增生的影响 |
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| 引用本文： | 甘云波,黄光英,张明敏. 针刺对脑缺血胶质增生的影响[J]. 武汉大学学报(医学版), 2007, 28(3): 329-331,I0002 |
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| 作者姓名： | 甘云波 黄光英 张明敏 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院中西医结合研究所,湖北,武汉,430030 |
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| 摘 要： | 目的:通过针刺对脑缺血后星形胶质细胞增殖进行干预,以探讨其对胶质增生的影响。方法:建立大鼠大脑中动脉阻塞(MCAO)再灌注模型,应用免疫组化和免疫印迹方法检测2 d和7 d后损伤侧海马细胞周期蛋白依赖性蛋白激酶4(CDK4)和胶质纤维酸性蛋白(GFAP)的表达。观察针刺对胶质增生的影响。结果:MCAO后2d,损伤侧海马CDK4蛋白表达明显增强(P<0.01),MCAO后7 d,损伤侧海马GFAP蛋白的表达明显增强(P<0.01),给予针刺后,可抑制CDK4蛋白和GFAP蛋白表达(P<0.01)。结论:针刺可抑制胶质增生,从而有可能为神经再生和功能重建提供更有利的生存环境。其机制可能与其调控细胞周期有关。
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| 关 键 词： | 针刺 星形胶质细胞 细胞周期 |
| 文章编号： | 1671-8852（2007）03-0329-03 |
| 修稿时间： | 2006-10-26 |
Effects of Acupuncture on Reactive Gliosis After Focal Cerebral Ischemia in Rats |
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| GAN Yunbo,HUANG Guangying,ZHANG Mingmin. Effects of Acupuncture on Reactive Gliosis After Focal Cerebral Ischemia in Rats[J]. Medical Journal of Wuhan University, 2007, 28(3): 329-331,I0002 |
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| Authors: | GAN Yunbo HUANG Guangying ZHANG Mingmin |
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| Affiliation: | Institute of Integrated Traditional Chinese and Western Medicine, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China |
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| Abstract: | Objective: To investigate the effects of acupuncture on astrocyte proliferation in hippocampus after focal cerebral ischemia reperfusion in rats.Methods: Ischemia/reperfusion injury was induced by temporary middle cerebral artery occlusion(MCAO).Acupuncture was administrated once a day for 2 and 7 days after MCAO,the expression level of CDK4 and glial fibrillary acidic protein(GFAP) was analyzed by Immunohistochemistry.Results: Two days after cerebral ischemia reperfusion,the expression of CDK4 increased significantly(P<0.01).And 7 days later,that of GFAP increased significantly(P<0.01) too.Acupuncture could inhibit the expression of CDK4 and GFAP(P<0.01).Conclusion: By regulating cell cycle,acupuncture may inhibit the glial proliferation,which could contribute to the regeneration of nerve and recovery of function. |
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| Keywords: | Acupuncture Astrocyte Cell cycle |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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