In vitro inducible nitric oxide synthesis inhibitors from Alismatis Rhizoma. |
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| Authors: | N Y Kim T H Kang H O Pae B M Choi H T Chung S W Myung Y S Song D H Sohn Y C Kim |
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| Affiliation: | College of Pharmacy, Wonkwang University, Iksan, Chonbuk, Korea. |
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| Abstract: | Bioassay-guided fractionation of an aqueous extract of Alismatis Rhizoma has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, alismol (1) and alisol B monoacetate (2), together with an inactive triterpene, alisol C monoacetate (3). Compounds 1 and 2 inhibited nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibitory effects of 1 and 2 on NO synthesis were partly due to suppression of iNOS mRNA expression as determined by Northern blotting. |
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