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Sequential G-banding and fluorescent in situ hybridization on peripheral blood,bone marrow,and amniotic fluid samples
Authors:Anne Wiktor  Regina Puskorius  Kristina Zubrickas  Daniel L. Van Dyke
Affiliation:Department of Medical Genetics, Henry Ford Health System, Detroit, Michigan
Abstract:We describe a method for fluorescent in situ hybridization (FISH) on previously G-banded slides and mounted coverslip preparations. The technique is successful on previously G-banded slides from peripheral blood and bone marrow specimens and on amniotic fluid samples from in situ harvests. Satellite sequence probes, whole chromosome paints, and unique sequence probes have all produced reliable results. Am. J. Med. Genet. 79:38–41, 1998. © 1998 Wiley-Liss, Inc.
Keywords:FISH  fluorescent in situ hybridization  sequential banding  G-banding
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