口腔鳞癌IV型胶原和层粘连蛋白的表达与颈淋巴结转移的关系

目的 探讨口腔鳞癌ＩＶ型胶原（ｃｏｌ ＩＶ）和层粘连蛋白（ＬＮ）的表达及其与临床病理参数的关系。方法 应用免疫组化ＳＡＢＣ法检测了３０例口腔鳞癌组织和５例人正常口腔膜组ＣｏｌⅣ和ＬＮ的表达，并用病理图像分析软件定量分析了两者的表达程度；对ＣｏｌⅣ和ＬＮ的表达与临床病理参数的关系进行了统计学分析。结果 ＣｏｌⅣ和ＬＮ的表达部位相同，主要分布于口腔粘膜和鳞癌组织基底膜；正常粘膜ＣｏｌⅣ和ＬＮ表达完整连

Expression of collagen IV and laminin in oral squamous cell carcinoma and its relationship with metastasis to neck lymph nodes

Objective To investigate the expression of collagen IV and laminin in oral squamous eell carcinoma and its correlation with clinicopathological parameters. Methods Immunohistochemical SABC methods was used to detect expression of collagen IV and laminin in 30 oral squamous cell carcinoma and 5 normal oral mucosa, the level of expression was analyzed quantitatively by image analysis software; Relationship between expression of collagen IV and laminin and differentiation, clinical stage, metastasis to neck lymph nodes was examined with Student t test.Results Collagen IV and laminin had similar expression location, which distributed in basement membrane of normal and cancer tissue; Complete and continuous expression could be seen in normal mucous tissue, however, their expression was destructed in some extent in cancer tissue; Expression of collagen IV and laminin showed no correlation with differentiation and clinical stage of cancer, but close relationship between expression and metastasis to neck lymph nodes was found. Conclusion Destruction of collagen IV and laminin is probably necessary to metastasis of oral squamous cell carcinoma to neck lymph nodes.