单因素考察和正交试验结合优选黄芪药材蛋白质提取工艺

目的:优选黄芪药材可溶性蛋白质的最佳提取工艺。方法:对黄芪药材蛋白质提取条件进行单因素考察,并采用L9(34)正交试验设计法,采用考马斯亮蓝染色法,以黄芪药材可溶性蛋白质含量为指标,考察料液比、pH值、提取温度、提取时间四个因素对可溶性蛋白质提取率的影响,从而确定黄芪药材蛋白质的最佳提取工艺。结果:建立了黄芪药材蛋白质的最佳提取工艺:向黄芪药材粉末中加入20倍量pH为12的蒸馏水,在温度30℃条件下回流提取10 min。结论:优化的提取工艺稳定、合理、可行,能正确反映黄芪药材中的蛋白质含量,为黄芪生物活性物质的进一步研究提供了实验依据。

Optimization Investigation of Extraction Technology of Protein in Astragali radix by Single Factor Test and Orthogonal Test

Objective：To optimize the extraction technology of protein in Astragali radix. Methods：The optimized extraction technology was investigated by using single factor test and orthogonal test with the content of water - soluble protein as the index for the first time which was determined by the method of coomassie brilliant blue staining. In this study, the effect of solid - liquid ratio, pH, temperature and time on the extraction rate was investigated. Results ： The optimized extraction technique of protein in As- tragali radix was established, that was pH 12, solid - liquid ratio 1 ： 20 and 10 min for extraction at the temperature of 30 ℃. Con- clusion：The optimal extraction condition was stable and reliable, and could provide an experimental basis for further study of the bioactive substance of Astragali radix.