| 白术水提物对酵母朊病毒[PSI^＋]治愈作用的定量研究 |
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| 引用本文： | 李辉,;窦爽,;张波,;卜祥龙,;钟正伟,;宋有涛.白术水提物对酵母朊病毒[PSI^＋]治愈作用的定量研究[J].中国人兽共患病杂志,2014(10):1024-1027. |
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| 作者姓名： | 李辉 ;窦爽 ;张波 ;卜祥龙 ;钟正伟 ;宋有涛 |
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| 作者单位： | [1]辽宁大学生命科学院,沈阳110036; [2]东港出入境检验检疫局,东港118300; [3]承德石油高等专科学校,承德067000 |
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| 基金项目： | 国家自然科学基金（No.30970152）资助 |
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| 摘 要： | 目的定量研究白术水提物对酵母朊病毒PSI^＋]的治愈作用。方法首先用含白术水提物的培养基培养酵母朊病毒PSI^＋]阳性菌株，初步评价白术的治愈作用。然后在细胞水平借助影印培养法和蛋白水平上使用半变性琼脂糖凝胶电泳结合蛋白免疫技术进一步验证白术水提物对酵母朊病毒PSI^＋]的治愈作用。结果白术水提物作用酵母朊病毒PSI^＋]后的治愈率为6％。结论白术水提物对酵母朊病毒PSI^＋]有一定的治愈作用。
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| 关 键 词： | 酵母朊病毒 白术水提物 半变性琼脂糖凝胶电泳 |
Curing effects evaluation of rhizoma atractylodis macrocephalae on yeast prion [PSI^＋] |
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| Institution: | LI Hui ,DOU Shuang ,ZHONG Bo,BU Xiang-long ,ZHONG Zheng-wei ,SONG You-tao (1. School of Life Science, Liaoning University, Shenyang 110036, China; 2. The Second Department of Supervision and Inspection, Donggang Entry-Exit Inspection and Quaranting Bureau, Donggang 118300, China;3. Department of Chemical Engineering, Chengde Petroleum College, Chengde 067000, China) |
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| Abstract: | Investigation of the effects of rhizome atractylodis macrocephalae on yeast prion PSI^＋]was conducted in this study. Liquid culture containing rhizome atractylodis macrocephalae aqueous extracts was applied to evaluate its therapeutic effects preliminarily. Then yeast replica plating together with Semi Denaturing Detergent Agarose Gel Electrophoresis com- bined with western blot were used to further confirm the results. Its showed that the cure rate of aqueous extracts of rhizome atractylodis macrocephalae on yease prion PSI^＋]could reach 60%. |
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| Keywords: | yeast prion aqueous extracts of rhizoma atractylodis macrocephalae Semi Denaturing Detergent Agarose Gel Electrophoresis |
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