黑龙江逊克地区森林革蜱斑点热立克次体DNA的检测

目的 调查黑龙江逊克地区蜱传斑点热自然疫源地,发现该地区蜱携带斑点热立克次体的种类。方法 采用斑点热立克次体ompA和gltA基因特异的PCR,检测该地区森林革蜱的DNA样本,并对扩得阳性产物进行测序和聚类分析。结果 从60只森林革蜱中检测有14只扩得斑点热立克次体ompA和gltA基因片段,阳性率为23.33%。随机选择2只蜱的阳性片段进行测序,二者同源性为100%,ompA基因序列与Rickettsia sp.JL-02同源性为99.30%, 与Rickettsia raoultii为99.18%。结论 黑龙江省逊克地区森林革蜱携带与Rickettsia sp.JL-02株亲缘关系相近的斑点热群立克次体。

Molecular detection of spotted fever group rickettsia in Dermacentor silvarum from the Xunke Area,China

ABSTRACT：To identify the prevalence and the distribution of ticks infected with spotted fever group rickettsiae （SFGR） in Xunke Area of Heilongjiang Province, China, partial outer membrane protein A gene （ompA） and citrate synthase gene （gltA） specific fragments were assessed using the PCR method. The positive products were sequenced. Result showed that the pres- ence of SFGR was 14 of 60 in detection Dermacentor silvarum cases, while the overall positive rate was 23.33 %. Its nucleotide sequence of ompA showed 99.3 % and 99.18~ similarity with nucleotide sequence of Rickettsia sp. JL-02 and Rickettsia rao- ultii respectively. And the evolutionary positions of SFGR species were similar with Rickettsia montana and Rickettsia massili- ae. It＇s concluded that the nature focus of tick-borne spotted fever did exist in the area of Xunke Area of Heilongjiang Province, China.