桑叶中芦丁含量的动态变化研究

目的：研究不同时期桑叶中芦丁的含量。方法 Kromasil C18柱（4.6 mm ×250 mm，5μm）；流动相：甲醇-0.5％磷酸水溶液（梯度洗脱），检测波长：358 nm；柱温：30℃；结果芦丁在0.1262~2.02μg范围内有良好的线性关系，r＝0.9999，平均回收率为97.88％，桑叶中芦丁含量最高在霜降前后。结论初步表明了桑叶中芦丁含量随季节变化的规律。

Study on the dynamic change of rutin content in mulberry leaves

Objective To study the content of rutin in different period of mulberry leaf. Methods The Kromasil C18 column（4. 6 mm × 250 mm,5μm）was used,and mobile phase was composed of methanol-0. 5% phosphoric acid solution （ gradient elution）,Detection wavelength was at 358 nm,the column temperature was at 30℃. Results The linear range of rutin was 0. 126 2~2. 02 μg（ r=0. 999 9）. The average recovery of rutin was 97. 88%,the highest content of rutin in mul-berry leaves before and after the frost. Conclusion Preliminary results suggested that rules of rutin in mulberry leaves sea-sonal changes.