HPLC法测定鼻炎糖浆中盐酸麻黄碱和盐酸伪麻黄碱的含量

目的：建立高效液相色谱法测定鼻炎糖浆中盐酸麻黄碱和盐酸伪麻黄碱含量的测定方法。方法采用Agilent Zorbax SB-C18色谱柱（4.6 mm ×250 mm，5μm），乙腈-0.2％磷酸溶液（含0.2％三乙胺）（2：98）为流动相，流速：1.0 mL ·min-1，检测波长206 nm，柱温：25℃。结果盐酸麻黄碱进样量在0.124~1.86μg范围内与峰面积呈良好线性（r＝0.9999），平均回收率为99.99％，RSD＝1.24％（n＝6）；盐酸伪麻黄碱进样量在0.1038~1.557μg范围内与峰面积呈良好线性（r＝0.9999），平均回收率为99.33％，RSD＝1.93％（n＝6）。结论该方法准确、重复性好，可用于鼻炎糖浆中盐酸麻黄碱和盐酸伪麻黄碱的测定。

Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Biyan Syrup by HPLC

Objective To establish an HPLC method for the determination of ephedrine hydrochloride and pseudo-ephedrine hydrochloride in Biyan Syrup. Methods The Agilent Zorbax SB-C18（4. 6 mm × 250 mm,5 μm）column was used. The mobile phase consisted of acetonitrile-0. 20% phosphoric acid（containing 0. 2% triethylamine）（2：98）with the flow rate of 1. 0 mL·min-1 . The detection wavelength was 206 nm and the column temperature was 25 ℃. Results The linear range of ephedrine hydrochloride and pseudoephedrine hydrochloride was 0. 124~1. 86μg（r=0. 999 9）and 0. 103 8~1. 557 μg（r=0. 999 9）with the average recovery of 99. 99%（RSD=1. 24%,n=6）and 99. 33%（RSD=1. 93%,n=6）,respectively. Conclusion The method was accurate and reproducible. It can be used for the determination of ephed-rine hydrochloride and pseudoephedrine hydrochloride in Biyan Syrup.