| 人X染色体α卫星DNA的定位及应用初探 |
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| 引用本文： | 裘宏琛 刘凤喜. 人X染色体α卫星DNA的定位及应用初探[J]. 中国医学科学院学报, 1991, 13(3): 203-207 |
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| 作者姓名： | 裘宏琛 刘凤喜 |
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| 作者单位： | 中国医学科学院基础医学研究所，北京医科大学第一附属医院，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，北京医科大学第一附属医院 北京，北京，北京 |
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| 摘 要： | 应用人X染色体α卫星DNA探针对正常男女及45,XO/47,XXX个体外周血淋巴细胞的原位杂交研究表明,在R显带的中期分裂相中,绝大部分杂交颗粒位于X染色体着丝粒区,即p11→q11区,在间期核内,则呈现与X染色体数目明显相关的银颗粒簇,其中44％位于核边缘区。本实验显示α卫星DNA的染色体区域定位及在具有不同X染色体数目间期核内分布的高度特异性。
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| 关 键 词： | α卫星DNA 原位杂交 R显带技术 |
Chromosomal Localization of Human X Chromosome Alphoid Satellite DNA by in Situ Hybridization and Its Preliminary Application |
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| Qiu Hongchen,et al. Chromosomal Localization of Human X Chromosome Alphoid Satellite DNA by in Situ Hybridization and Its Preliminary Application[J]. Acta Academiae Medicinae Sinicae, 1991, 13(3): 203-207 |
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| Authors: | Qiu Hongchen et al |
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| Affiliation: | Institute of Basic Medical Sciences, Beijing. |
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| Abstract: | In situ hybridization of human X chromosome-specific alphoid satellite DNA to human lymphocyte metaphase and interphase nuclei was carried out. The results showed that the probe hybridized with high specificity to the centromeric region at p11----q11 of the X chromosome. The number of silver grain clusters in the interphase nuclei was correlated with that of X chromosomes in the cells. 44% of the clusters were located near the nuclear membrane, the usual location of X chromatin (Barr body). The hybridized chromosomes were also R-banded with BrdU treatment, and the significance of this work was briefly discussed. |
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