尼尔雌醇作用下去势雌兔骨组织形态计量学改变

目的：评估尼尔雌醇对绝经后骨质疏松症的疗效，并探讨其机理。方法：将３０只雌性新西兰兔随机分成３组（每组１０只）：治疗组［摘除卵巢＋尼尔雌醇（每月２ｍｇ）］、模型组（摘除卵巢）和正常对照组（假手术），６个月后处死取材，并在处死前行四环素标记，ＶＩＤＡＳ图象分析系统下作骨组织形态计量学测定。结果：治疗组的单位体积骨小梁骨量（ＢＶ／ＴＶ）、相对类骨质量（ＯＶ／ＢＶ）、成骨细胞指数（ＯＢＩ）和组织水平的骨形成速率［ＢＦＲ（Ｔ）］明显高于模型组和（或）正常对照组。结论：尼尔雌醇能增加成骨细胞数量，可能是一种成骨细胞募集和（或）增殖的刺激剂。

Histomorphometric Study of the Effect of Nylestriol on Bone Turnover in Ovariectomized Rabbits

Objective: To assess the effect of Nylestriol on postmenopausal osteoporosis in an attempt to reveal its mechanism. Methods: Thirty seven month old female New Zealand white rabbits were randomized into 3 groups (10 rabbits per group): treatment group ovariectomized (OVX) nylestriol (2 mg, monthly)], OVX control group (OVX vehicle) and normal control group (sham operation vehicle). Six months later, all rabbits were subjected to tetracycline labelling and then were killed. Under VIDAS system, bone turnover was assessed by bone histomorphometry. Results: Bone volume trabecular (BV/TV),relative osteoid volume (OV/BV), osteoblastic index (OBI) and bone formation rate at tissue level increased in treatment group compared with OVX control group and (or ) sham control group. Conclusions: Nylestriol can increase the number of osteoblast, which resulted in more bone matrix production and consequently converted the bone turnover of OVX rabbits to a positive balance situation. It indicated that Nylestriol was probably a stimulator for osteoblast recruitment and (or ) proliferation.