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| ATP依赖性生物发光测定L型细菌对药物的敏感性 | |
| 引用本文： | 唐银,文斌,李宪,郭思建,颜学军. ATP依赖性生物发光测定L型细菌对药物的敏感性[J]. 中南大学学报(医学版), 2001, 26(1): 89-91 |
| 作者姓名： | 唐银 文斌 李宪 郭思建 颜学军 |
| 作者单位： | 1. 中南大学湘雅医院检验科 2. 中南大学湘雅医院肾病科 3. 中南大学湘雅医学院微生物学教研室 |
| 摘 要： | 用生物发光BL技术检测 111株L型细菌对药物的敏感性 ;其中包括L型金黄色葡萄球菌、L型肠间链球菌、L型肺炎克雷伯氏菌、绿脓杆菌及L型沙门氏菌属。同时用BL法和K -B法对比检测对 5种抗生素的敏感性。结果示 :平均发光值 (CPM .S - 1)≤ 30 %为敏感 ,>35 %为耐药。本法与K -B法符合率为 95 .3% ,变异率为 10 %。提示B -L法直接测定L型菌的药敏性具有实用价值。 |
| 关 键 词： | 生物测定 三磷酸腺苷 药物敏感性 微生物 抗生素类 |
| 文章编号： | 1000-5625(2001)01-0089-03 |
| 修稿时间： | 2000-04-12 |
Using L-form bacterium ATP bioluminescence assay for rapid tesing L-form bacterial susceptibility |
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| TANG Yin ,WEN Bin ,LI Xian ,YAN Xue jun ,GUO Si jian. Using L-form bacterium ATP bioluminescence assay for rapid tesing L-form bacterial susceptibility[J]. Journal of Central South University. Medical sciences, 2001, 26(1): 89-91 | |
| Authors: | TANG Yin WEN Bin LI Xian YAN Xue jun GUO Si jian |
| Affiliation: | 1Department of Clinical Laboratory, Xiangya Hospital, Central South University(Changsha 410008); 2Department of Nephrosis, Xiangya Hospital, Central South University(Chan |
| Abstract: | Adenosine triphosphate is a kind of necessary metabolites in living cells. The authors detected ATP contents by using bioluminescence method in 111 strains of L form bacteria after exposing to 5 kinds of antibiotics. The results showed that the mean value of CPM was less than (35±10.2)%·s -1 . Thus, the value could be acted as a critical concentration between susceptibility and resistance. The conincidence rate of this method and K B method was 95.3%. It indicates that the bioluminescence method has a high sensitivity. It can be used to detect L form bacterium drug susceptibility quickly and may play an important role for choosing the antibiotics. |
| Keywords: | biological assay adenosine thriphosphate drug susceptibility microbial antibioltics |
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