| 羧甲基壳聚糖与阿霉素联合用药的体外抗肿瘤作用 |
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| 引用本文： | 杨靖亚,王晓霁,张建. 羧甲基壳聚糖与阿霉素联合用药的体外抗肿瘤作用[J]. 中国临床药理学与治疗学, 2009, 0(12): 1253-1258 |
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| 作者姓名： | 杨靖亚 王晓霁 张建 |
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| 作者单位： | 上海海洋大学食品学院,上海201306 |
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| 基金项目： | 上海市教育委员会科研创新项目（09YZ265） |
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| 摘 要： | 目的：探讨羧甲基壳聚糖与阿霉素联合应用对人宫颈癌Hela细胞及人结肠腺瘤LS174-T细胞增殖的影响。方法：采用MTT比色法测定单独和合用羧甲基壳聚糖与阿霉素对癌细胞的生长抑制作用。应用金氏公式进行联合用药分析。结果：羧甲基壳聚糖和阿霉素均可有效地抑制癌细胞生长，且同时给药对癌细胞可产生单纯相加至增强的协同杀伤效果。序贯给药法中先给阿霉素后给羧甲基壳聚糖结果为协同作用，先给羧甲基壳聚糖后给阿霉素为拮抗作用。结论：羧甲基壳聚糖与阿霉素同时联合用药可产生协同作用，序贯联合用药产生单向协同作用。
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| 关 键 词： | 羧甲基壳聚糖 阿霉素 MTT法 Hela LS174-T 药物协同作用 |
Antitumor effect of combination of carboxymethyl-chitosan and adriamycin in vitro |
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| YANG Jing-ya,WANG Xiao-ji,ZHANG Jian. Antitumor effect of combination of carboxymethyl-chitosan and adriamycin in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 0(12): 1253-1258 |
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| Authors: | YANG Jing-ya WANG Xiao-ji ZHANG Jian |
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| Affiliation: | (College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China) |
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| Abstract: | AIM： To investigate the effects of carboxymethyl chitosan （ CM-CTS） and adriamycin （ADM） on the proliferation of human cervical carcinoma cell line Hela and human colon cancer cells KS174- T in vitro. METHODS： The anti-proliferation activities of two drugs on tumor cells were evaluated by MTT assay. The jin＇s formula was used to analyze the experimental data. RESULTS： Both CM-CTS and ADM could inhibit tumor cell proliferation and an additional synergistic inhibitory effect could be improved when two drugs were combined. In sequential test, administration of ADM first followed by CM-CTS had a synergistic effect; but administration of CM-CTS first followed by ADM produced an antagonistic effect. CONCLUSION： Combined treatment with CM-CTS and ADM produces synergistic effects. Sequential administration of CM-CTS and ADM produces a unidirectional synergistic effect. |
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| Keywords: | carboxymethyl-chitosan adriamycin MTT assay Hela LS174-T synergistic action of drug |
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